Publication Name	World Journal of Gastroenterology
Manuscript ID	47640
Country	China

Received	2019-03-26 02:20
Peer-Review Started	2019-03-26 04:55
To Make the First Decision	2019-05-27 00:35
Return for Revision	2019-05-31 01:50
Revised	2019-06-13 15:31
Second Decision	2019-07-05 10:26
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2019-07-05 23:48
Articles in Press	2019-07-05 23:48
Publication Fee Transferred
Edit the Manuscript by Language Editor	2019-07-15 03:35
Typeset the Manuscript	2019-08-05 08:32
Publish the Manuscript Online	2019-08-07 07:09

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access	This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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Category	Gastroenterology & Hepatology
Manuscript Type	Retrospective Cohort Study
Article Title	Liquid biopsy for non-invasive assessment of liver injury in hepatitis B patients
Manuscript Source	Unsolicited Manuscript
All Author List	Wen-Ying Xia, Li Gao, Er-Hei Dai, Dan Chen, Er-Fu Xie, Li Yang, Shi-Chang Zhang, Bing-Feng Zhang, Jian Xu and Shi-Yang Pan
ORCID	Author(s) ORCID Number Wen-Ying Xia http://orcid.org/0000-0003-2359-1379 Li Gao http://orcid.org/0000-0002-9762-448X Er-Hei Dai http://orcid.org/0000-0001-8835-6199 Dan Chen http://orcid.org/0000-0003-1141-3890 Er-Fu Xie http://orcid.org/0000-0002-6787-7646 Li Yang http://orcid.org/0000-0002-8762-2923 Shi-Chang Zhang http://orcid.org/0000-0002-6587-2518 Bing-Feng Zhang http://orcid.org/0000-0003-0240-3447 Jian Xu http://orcid.org/0000-0003-2032-251X Shi-Yang Pan http://orcid.org/0000-0001-9320-6988
Funding Agency and Grant Number	Funding Agency Grant Number National Natural Science Foundation of China 81672100 National Natural Science Foundation of China 81371894 Key Laboratory for Laboratory Medicine of Jiangsu Province of China ZDXKB2016005 Jiangsu Provincial Commission of Health and Family Planning H201609
Corresponding Author	Shi-Yang Pan, MD, Professor, Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, Guangzhou Road No. 300, Nanjing 210029, Jiangsu Province, China. sypan@njmu.edu.cn
Key Words	Liquid biopsy; plasma DNA; Hepatitis B; Alanine aminotransferase; Duplex real-time quantitative polymerase chain reaction;
Core Tip	Our study quantified cell free DNA combined with other serum biomarker as a liquid biopsy-based method to assess liver injury in hepatitis B patients. A cohort of 663 subjects including 313 hepatitis B patients and 350 healthy controls were enrolled. The ultrasound-guided liver biopsies followed by histopathological assessments were performed for the 263 chronic hepatitis B patients to determine the degree of liver injury. Cell-free DNA was quantified using a novel duplex real-time polymerase chain reaction assay. Our results demonstrated that the combination of plasma DNA, serum alanine aminotransferase and bilirubin could be a candidate liquid biopsy for non-invasive assessment of liver injury in hepatitis B patients.
Publish Date	2019-08-07 07:09
Citation	Xia WY, Gao L, Dai EH, Chen D, Xie EF, Yang L, Zhang SC, Zhang BF, Xu J, Pan SY. Liquid biopsy for non-invasive assessment of liver injury in hepatitis B patients. World J Gastroenterol 2019; 25(29): 3985-3995
URL	https://www.wjgnet.com/1007-9327/full/v25/i29/3985.htm
DOI	https://dx.doi.org/10.3748/wjg.v25.i29.3985

Full Article (PDF)	WJG-25-3985.pdf
Full Article (Word)	WJG-25-3985.docx
STROBE Statement	47640-STROBE-Statement-revision.pdf
Manuscript File	47640-Review_Edited.doc
Answering Reviewers	47640-Answering reviewers.pdf
Audio Core Tip	47640-Audio core tip.mp3
Biostatistics Review Certificate	47640-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	47640-Conflict-of-interest statement.pdf
Copyright License Agreement	47640-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	47640-Grant application form(s).pdf
Signed Informed Consent Form(s) or Document(s)	47640-Informed consent statement.pdf
Institutional Review Board Approval Form or Document	47640-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate	47640-Language certificate.pdf
Peer-review Report	47640-Peer-review(s).pdf
Scientific Misconduct Check	47640-Scientific misconduct check.pdf
Scientific Editor Work List	47640-Scientific editor work list.pdf