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Articles Published Processes
12/30/2019 1:55:29 AM | Browse: 824 | Download: 1252
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Received |
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2019-04-01 01:55 |
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Peer-Review Started |
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2019-04-03 09:49 |
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To Make the First Decision |
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2019-08-28 05:22 |
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Return for Revision |
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2019-09-02 09:04 |
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Revised |
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2019-09-30 10:30 |
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Second Decision |
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2019-12-13 11:31 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Executive Editor-in-Chief |
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2019-12-15 06:25 |
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Articles in Press |
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2019-12-15 06:25 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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Typeset the Manuscript |
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2019-12-25 01:29 |
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Publish the Manuscript Online |
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2019-12-30 01:55 |
| ISSN |
1948-0210 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Cell Biology |
| Manuscript Type |
Review |
| Article Title |
Inducing human induced pluripotent stem cell differentiation through embryoid bodies: A practical and stable approach
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| Manuscript Source |
Invited Manuscript |
| All Author List |
Ning-Ning Guo, Li-Ping Liu, Yun-Wen Zheng and Yu-Mei Li |
| ORCID |
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| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| National Natural Science Foundation of China |
81573053 |
| National Natural Science Foundation of China |
81770621 |
| Ministry of Education, Culture, Sports, Science, and Technology of Japan, KAKENHI |
16K15604 |
| Ministry of Education, Culture, Sports, Science, and Technology of Japan, KAKENHI |
18H02866 |
| Natural Science Foundation of Jiangsu Province |
BK20180281 |
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| Corresponding Author |
Yun-Wen Zheng, PhD, Associate Professor, Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, University of Tsukuba Faculty of Medicine, Tennodai 1-1-1, Tsukuba 305-8575, Ibaraki, Japan. ywzheng@md.tsukuba.ac.jp |
| Key Words |
Induced pluripotent stem cells; Suspension culture; Embryoid body; Early prediction; Committed differentiation; Heterogeneity |
| Core Tip |
Several factors lead to uncertain and unpredictable effects in iPSC culture and differentiation, which increase the risk of differentiation failure and cause the wastage of resources. Meanwhile, embryoid body (EB)-mediated differentiation is critical for iPSC-derived cell generation. Here, we summarize and update the approaches of EB formation in recent years, together with our experiences in the laboratory, to demonstrate the key characteristics and practical differentiation of hiPSCs in applications. |
| Publish Date |
2019-12-30 01:55 |
| Citation |
Guo NN, Liu LP, Zheng YW, Li YM. Inducing human induced pluripotent stem cell differentiation through embryoid bodies: A practical and stable approach. World J Stem Cells 2020; 12(1): 25-34 |
| URL |
https://www.wjgnet.com/1948-0210/full/v12/i1/25.htm |
| DOI |
https://dx.doi.org/10.4252/wjsc.v12.i1.25 |
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