Publication Name	World Journal of Gastroenterology
Manuscript ID	49124
Country	Japan

Received	2019-05-17 00:41
Peer-Review Started	2019-05-17 06:27
To Make the First Decision	2019-06-17 00:37
Return for Revision	2019-06-21 00:48
Revised	2019-07-04 10:00
Second Decision	2019-07-12 10:16
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2019-07-19 15:12
Articles in Press	2019-07-19 15:12
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2019-08-27 06:33
Publish the Manuscript Online	2019-08-28 12:23

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access	This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Copyright	© The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
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Category	Gastroenterology & Hepatology
Manuscript Type	Minireviews
Article Title	Exploring the hepatitis C virus genome using single molecule real-time sequencing
Manuscript Source	Invited Manuscript
All Author List	Haruhiko Takeda, Taiki Yamashita, Yoshihide Ueda and Akihiro Sekine
ORCID	Author(s) ORCID Number Haruhiko Takeda http://orcid.org/0000-0002-8954-9133 Taiki Yamashita http://orcid.org/0000-0002-5465-534X Yoshihide Ueda http://orcid.org/0000-0003-3196-3494 Akihiro Sekine http://orcid.org/0000-0003-3313-4331
Funding Agency and Grant Number
Corresponding Author	Akihiro Sekine, PhD, Professor, Department of Omics-based Medicine, Center for Preventive Medical Science, Chiba University, 1-8-1, Inohana, Chuo-ku, Chiba 260-0856, Japan. sekine.akihiro@chiba-u.jp
Key Words	Third generation sequencing; PacBio RSII; Single molecule real-time sequencing; Hepatitis C virus; Resistance-associated substitution; Nanopore sequencer
Core Tip	Single molecular real-time (SMRT) sequencing, also called third-generation sequencing, is a novel sequencing technique capable of generating extremely long contiguous sequence reads. The advantages of long read sequencing enable accurate determination of the haplotypes of infected viral clones. We introduce the novel third-generation sequencers PacBio RSII/Sequel systems, compare them with conventional next-generation sequencers, and summarize previous studies in which SMRT sequencing technology has been applied for hepatitis C virus genome analysis.
Publish Date	2019-08-28 12:23
Citation	Takeda H, Yamashita T, Ueda Y, Sekine A. Exploring the hepatitis C virus genome using single molecule real-time sequencing. World J Gastroenterol 2019; 25(32): 4661-4672
URL	https://www.wjgnet.com/1007-9327/full/v25/i32/4661.htm
DOI	https://dx.doi.org/10.3748/wjg.v25.i32.4661

Full Article (PDF)	WJG-25-4661.pdf
Full Article (Word)	WJG-25-4661.docx
Manuscript File	49124-Review.docx
Answering Reviewers	49124-Answering reviewers.pdf
Audio Core Tip	49124-Audio core tip.mp3
Conflict-of-Interest Disclosure Form	49124-Conflict-of-interest statement.pdf
Copyright License Agreement	49124-Copyright license agreement.pdf
Non-Native Speakers of English Editing Certificate	49124-Language certificate.pdf
Peer-review Report	49124-Peer-review(s).pdf
Scientific Misconduct Check	49124-Scientific misconduct check.pdf
Scientific Editor Work List	49124-Scientific editor work list.pdf
CrossCheck Report	49124-CrossCheck Report.pdf