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Articles Published Processes
11/12/2019 9:28:30 AM | Browse: 686 | Download: 1220
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Received |
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2019-08-05 11:16 |
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Peer-Review Started |
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2019-08-05 11:16 |
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To Make the First Decision |
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2019-08-21 07:16 |
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Return for Revision |
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2019-08-21 08:49 |
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Revised |
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2019-09-27 06:12 |
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Second Decision |
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2019-10-12 06:28 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Executive Editor-in-Chief |
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2019-10-15 21:09 |
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Articles in Press |
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2019-10-15 21:09 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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2019-10-25 17:57 |
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Typeset the Manuscript |
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2019-11-05 12:12 |
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Publish the Manuscript Online |
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2019-11-12 09:28 |
| ISSN |
2222-0682 (online) |
| Open Access |
This is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Medical Laboratory Technology |
| Manuscript Type |
Basic Study |
| Article Title |
DNA extraction from archived hematoxylin and eosin stained tissue slides for downstream molecular analysis
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| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Pushkal Sinduvadi Ramesh, Venkatesh Madegowda, Suprith Kumar, Shailashree Narasimha, Parichay S R, Nandini Nandish Manoli and Devananda Devegowda |
| ORCID |
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| Funding Agency and Grant Number |
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| Corresponding Author |
Devananda Devegowda, MSc, PhD, Assistant Professor, Center of Excellence in Molecular Biology and Regenerative Medicine, Department of Biochemistry, JSS Medical College, JSS Academy of Higher Education and Research, Mysuru 570015, India. devanandd@jssuni.edu.in |
| Key Words |
DNA extraction; Hematoxylin and eosin tissue slide; Molecular analysis; Polymerase chain reaction amplification; Deparaffinization; |
| Core Tip |
Here in our study, we discuss a step by step procedure and results for the extraction of PCR friendly DNA from archived hematoxylin and eosin stained tissue slides. Such extracted DNA has the potential to be used for further molecular analyses such as mutation studies, whole genome sequencing and even to identify difference in gene signatures between diseased and normal state. Our protocol is simple, cost-effective and can be performed in a basic molecular biology lab with most common reagents. |
| Publish Date |
2019-11-12 09:28 |
| Citation |
Ramesh PS, Madegowda V, Kumar S, Narasimha S, S R P, Manoli NN, Devegowda D. DNA extraction from archived hematoxylin and eosin stained tissue slides for downstream molecular analysis. World J Methodol 2019; 9(3): 32-43 |
| URL |
https://www.wjgnet.com/2222-0682/full/v9/i3/32.htm |
| DOI |
https://dx.doi.org/10.5662/wjm.v9.i3.32 |
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