Publication Name	World Journal of Gastroenterology
Manuscript ID	5235
Country	Taiwan

Received	2013-08-24 08:42
Peer-Review Started	2013-08-24 10:42
To Make the First Decision	2013-09-02 16:24
Return for Revision	2013-09-03 21:35
Revised	2013-09-17 17:28
Second Decision	2013-10-21 17:39
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2013-10-22 07:45
Articles in Press
Publication Fee Transferred
Edit the Manuscript by Language Editor	2014-02-09 11:12
Typeset the Manuscript	2014-03-11 11:55
Publish the Manuscript Online	2014-03-27 12:04

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access
Copyright
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Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Gastroenterology & Hepatology
Manuscript Type	Autobiography
Article Title	Detection of genotypic clarithromycin-resistant Helicobacter pylori by string tests
Manuscript Source	Invited Manuscript
All Author List	Jeng-Yih Wu, Sophie S W Wang, Yi-Chern Lee, Yoshio Yamaoka, David Y Graham, Chang-Ming Jan, Wen-Ming Wang and Deng-Chyang Wu
Funding Agency and Grant Number	Funding Agency Grant Number National Science Council of Republic of China NSC96-3111-P-042A-004-Y National Science Council of Republic of China NSC97-2314-B-037-047-MY3 Kaohsiung Medical University Hospital KMUH97-7R32 Kaohsiung Medical University Hospital KMUH97-7G49
Corresponding Author	Deng-Chyang Wu, MD, PhD, Division of Internal Medicine, Kaohsiung Municipal Hsiao-Kang Hospital, 482 Shanming Rd, Hsiaogang Dist, Kaohsiung 807, Taiwan. dechwu@yahoo.com
Key Words	Helicobacter pylori; String test; Clarithromycin resistance; Polymerase chain reaction-restriction fragment length polymorphism
Core Tip	According to region, antibiotic resistance is mostly detected by culture of endoscopic biopsy. The study aimed to detect genotypic clarithromycin resistance by string tests. Amplified 23S rRNA from strings was digested by restriction enzymes to discriminate A2142G or A2143G mutations conferring clarithromycin resistance. Culture was also done to compare genotypic and phenotypic resistance. Sensitivity and specificity of the method were 66.7% and 97.3%, respectively. Positive and negative predictive values were 80% and 94.7%, respectively. Our study demonstrates that the string test, rather than endoscopic biopsy culture, could provide an option for molecular analysis in future.
Publish Date	2014-03-27 12:04
Citation	Wu JY, Wang SSW, Lee YC, Yamaoka Y, Graham DY, Jan CM, Wang WM, Wu DC. Detection of genotypic clarithromycin-resistant Helicobacter pylori by string tests. World J Gastroenterol 2014; 20(12): 3343-3349
URL	http://www.wjgnet.com/1007-9327/full/v20/i12/3343.htm
DOI	http://dx.doi.org/10.3748/wjg.v20.i12.3343

Full Article (PDF)	WJG-20-3343.pdf
Full Article (Word)	WJG-20-3343.doc
Manuscript File	5235-Review.doc
Answering Reviewers	5235-Answering reviewers.pdf
Copyright License Agreement	5235-Copyright assignment.pdf
Peer-review Report	5235-Peer review(s).pdf
Scientific Editor Work List	5235-Scientific editor work list.doc