Publication Name	World Journal of Diabetes
Manuscript ID	5539
Country	Canada

Received	2013-09-13 10:29
Peer-Review Started	2013-09-13 15:02
To Make the First Decision	2013-11-19 13:01
Return for Revision	2013-11-20 09:25
Revised	2013-11-27 00:33
Second Decision	2013-12-16 13:47
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2013-12-16 13:52
Articles in Press
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2014-02-15 16:52
Publish the Manuscript Online	2014-02-19 15:19

ISSN	1948-9358 (online)
Open Access
Copyright
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Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Research & Experimental Medicine
Manuscript Type	Autobiography
Article Title	Identification and differentiation of PDX1 β-cell progenitors within the human pancreatic epithelium
Manuscript Source	Invited Manuscript
All Author List	Karen L Seeberger, Sarah J Anderson, Cara E Ellis, Telford Y Yeung and Gregory S Korbutt
Funding Agency and Grant Number	Funding Agency Grant Number Canadian Institutes of Health Research MOP8030 Alberta Diabetes Institute
Corresponding Author	Gregory S Korbutt, Professor, Alberta Diabetes Institute, 5-002 Li Ka Shing Center, University of Alberta, Edmonton, 116 St. and 85 Ave, Alberta, T6G 2E1, Canada. korbutt@ualberta.ca
Key Words	Differentiation; Epithelial; Epithelial-mesenchymal transition; Mesenchymal; PDX1; Insulin; Progenitor; Vimentin
Core Tip	Previously, we demonstrated that mesenchymal stem cells could be expanded from human endocrine and non-endocrine pancreas cell fractions in vitro. However, we were unable to complete cell differentiation of mesenchymal cell intermediates to functional endocrine cells. In this study we utilized a novel cell culture medium to prevent epithelial cell de-differentiation and mesenchymal cell expansion. After epithelial cell expansion in this medium cells were differentiated via our previously described protocol and we confirmed by lineage tracing, flow cytometry, immunostaining and real-time polymerase chain reaction that islet progenitors reside in the pancreatic epithelium and are not derived via a mesenchymal cell intermediate.
Publish Date	2014-02-19 15:19
Citation	Seeberger KL, Anderson SL, Ellis CE, Yeung TY, Korbutt GS. Identification and differentiation of PDX1 β-cell progenitors within the human pancreatic epithelium. World J Diabetes 2014; 5(1): 59-68 Available from: URL: http://www.wjgnet.com/1948-9358/ full/v5/i1/59.htm DOI: http://dx.doi.org/10.4239/wjd.v5.i1.59
URL	http://www.wjgnet.com/1948-9358/full/v5/i1/59.htm
DOI	http://dx.doi.org/10.4239/wjd.v5.i1.59

Full Article (PDF)	WJD-5-59.pdf
Full Article (Word)	WJD-5-59.doc
Manuscript File	5539-Review.doc
Answering Reviewers	5539-Answering reviewers.pdf
Copyright License Agreement	5539-Copyright assignment.pdf
Institutional Review Board Approval Form or Document	Ethics_Committee_20130913060601.pdf
Peer-review Report	5539-Peer review(s).pdf
Scientific Editor Work List	5539-Scientific editor work list.doc