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9/1/2014 12:00:00 PM | Browse: 571 | Download: 468
Publication Name World Journal of Diabetes
Manuscript ID 5539
Country/Territory Canada
2013-09-13 10:29
Peer-Review Started
2013-09-13 15:02
To Make the First Decision
2013-11-19 13:01
Return for Revision
2013-11-20 09:25
2013-11-27 00:33
Second Decision
2013-12-16 13:47
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief
2013-12-16 13:52
Articles in Press
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript
2014-02-15 16:52
Publish the Manuscript Online
2014-02-19 15:19
ISSN 1948-9358 (online)
Open Access
Article Reprints For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website http://www.wjgnet.com
Category Research and Experimental Medicine
Manuscript Type Autobiography
Article Title Identification and differentiation of PDX1 β-cell progenitors within the human pancreatic epithelium
Manuscript Source Invited Manuscript
All Author List Karen L Seeberger, Sarah J Anderson, Cara E Ellis, Telford Y Yeung and Gregory S Korbutt
Funding Agency and Grant Number
Funding Agency Grant Number
Canadian Institutes of Health Research MOP8030
Alberta Diabetes Institute
Corresponding author Gregory S Korbutt, Professor, Alberta Diabetes Institute, 5-002 Li Ka Shing Center, University of Alberta, Edmonton, 116 St. and 85 Ave, Alberta, T6G 2E1, Canada. korbutt@ualberta.ca
Keywords Differentiation; Epithelial; Epithelial-mesenchymal transition; Mesenchymal; PDX1; Insulin; Progenitor; Vimentin
Core Tip Previously, we demonstrated that mesenchymal stem cells could be expanded from human endocrine and non-endocrine pancreas cell fractions in vitro. However, we were unable to complete cell differentiation of mesenchymal cell intermediates to functional endocrine cells. In this study we utilized a novel cell culture medium to prevent epithelial cell de-differentiation and mesenchymal cell expansion. After epithelial cell expansion in this medium cells were differentiated via our previously described protocol and we confirmed by lineage tracing, flow cytometry, immunostaining and real-time polymerase chain reaction that islet progenitors reside in the pancreatic epithelium and are not derived via a mesenchymal cell intermediate.
Publish Date 2014-02-19 15:19
Citation Seeberger KL, Anderson SL, Ellis CE, Yeung TY, Korbutt GS. Identification and differentiation of PDX1 β-cell progenitors within the human pancreatic epithelium. World J Diabetes 2014; 5(1): 59-68 Available from: URL: http://www.wjgnet.com/1948-9358/ full/v5/i1/59.htm DOI: http://dx.doi.org/10.4239/wjd.v5.i1.59
Url http://www.wjgnet.com/1948-9358/full/v5/i1/59.htm
DOI http://dx.doi.org/10.4239/wjd.v5.i1.59
Full Article (PDF) WJD-5-59.pdf
Full Article (Word) WJD-5-59.doc
Manuscript File 5539-Review.doc
Answering Reviewers 5539-Answering reviewers.pdf
Copyright License Agreement 5539-Copyright assignment.pdf
Institutional Review Board Approval Form or Document Ethics_Committee_20130913060601.pdf
Peer-review Report 5539-Peer review(s).pdf
Scientific Editor Work List 5539-Scientific editor work list.doc