Publication Name	World Journal of Gastroenterology
Manuscript ID	58505
Country	India

Received	2020-07-25 09:28
Peer-Review Started	2020-07-25 09:28
To Make the First Decision
Return for Revision	2020-09-14 04:33
Revised	2020-09-28 19:29
Second Decision	2020-11-09 09:34
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2020-11-12 22:23
Articles in Press	2020-11-12 22:23
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2020-12-02 05:27
Publish the Manuscript Online	2020-12-04 06:04

ISSN	1007-9327 (print) and 2219-2840 (online)
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Category	Gastroenterology & Hepatology
Manuscript Type	Basic Study
Article Title	Nimbolide inhibits tumor growth by restoring hepatic tight junction protein expression and reduced inflammation in an experimental hepatocarcinogenesis
Manuscript Source	Invited Manuscript
All Author List	Amit Kumar Ram, Balasubramaniyan Vairappan and BH Srinivas
ORCID	Author(s) ORCID Number Amit Kumar Ram http://orcid.org/0000-0002-9816-0875 Balasubramaniyan Vairappan http://orcid.org/0000-0003-1708-4864 BH Srinivas http://orcid.org/0000-0002-9619-6719
Funding Agency and Grant Number	Funding Agency Grant Number JIPMER intramural research grant; Indian Council of Medical Research (ICMR), New Delhi, India No. 3/1/3 J.R.F.-2016/LS/HRD Department of Biotechnology, Government of India No. 102/IFD/SAN/22/2013-14
Corresponding Author	Balasubramaniyan Vairappan, PhD, Associate Professor, Liver Diseases Research Lab, Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Dhanvantari Nagar, Dhanvantari Nagar, Puducherry 605006, India. balasubramaniyan.v@jipmer.edu.in
Key Words	Hepatocellular carcinoma; Nimbolide; Tight junction; Inflammation; Oxidative stress; Zonula occludens 1 associated nucleic acid binding protein
Core Tip	The effects of nimbolide on tight junction (TJ) proteins, cell cycle progression, and hepatic inflammation in diethylnitrosamine (DEN) and N-nitrosomorpholine (NMOR) induced hepatocellular carcinoma (HCC) mouse model is unknown. This study revealed for the first time that nimbolide suppresses tumor growth by restoring hepatic TJ proteins, inhibiting cell proliferation and cell cycle progression, and attenuating inflammation and oxidative stress in HCC mice. Moreover, nimbolide showed a modulatory effect on zonula occludens 1, nuclear factor of kappa light polypeptide gene enhancer in B-cells and tumor necrosis factor alpha proteins confirmed by in silico analysis. All the above results clarified that nimbolide abolished DEN and NMOR induced hepatocarcinogenesis and suggested that nimbolide could be a future potential drug candidate for the treatment of human HCC.
Publish Date	2020-12-04 06:04
Citation	Ram AK, Vairappan B, Srinivas BH. Nimbolide inhibits tumor growth by restoring hepatic tight junction protein expression and reduced inflammation in an experimental hepatocarcinogenesis. World J Gastroenterol 2020; 26(45): 7131-7152
URL	https://www.wjgnet.com/1007-9327/full/v26/i45/7131.htm
DOI	https://dx.doi.org/10.3748/wjg.v26.i45.7131

Full Article (PDF)	WJG-26-7131.pdf
Full Article (Word)	WJG-26-7131.docx
Manuscript File	58505_Auto_Edited.docx
Answering Reviewers	58505-Answering reviewers.pdf
Audio Core Tip	58505-Audio core tip.m4a
Biostatistics Review Certificate	58505-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	58505-Conflict-of-interest statement.pdf
Copyright License Agreement	58505-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	58505-Grant application form(s).pdf
Institutional Animal Care and Use Committee Approval Form or Document	58505-Institutional animal care and use committee statement.pdf
Institutional Review Board Approval Form or Document	58505-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate	58505-Language certificate.pdf
Supplementary Material	58505-Supplementary material.pdf
Peer-review Report	58505-Peer-review(s).pdf
Scientific Misconduct Check	58505-Bing-Yan JP-1.png
Scientific Misconduct Check	58505-Bing-Fan JR-2.png
Scientific Misconduct Check	58505-Scientific misconduct check.pdf
Scientific Editor Work List	58505-Scientific editor work list.pdf
CrossCheck Report	58505-CrossCheck Report.pdf
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