| ISSN |
1948-0210 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Neurosciences |
| Manuscript Type |
Basic Study |
| Article Title |
Disease modifying treatment of spinal cord injury with directly reprogrammed neural precursor cells in non-human primates
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Vladimir P Baklaushev, Oleg V Durov, Vladimir A Kalsin, Eugene V Gulaev, Sergey V Kim, Ilya L Gubskiy, Veronika A Revkova, Ekaterina M Samoilova, Pavel A Melnikov, Dzhina D Karal-Ogly, Sergey V Orlov, Alexander V Troitskiy, Vladimir P Chekhonin, Alexander V Averyanov and Jan-Eric Ahlfors |
| ORCID |
|
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Russian Science Foundation |
16-15-10432 |
|
| Corresponding Author |
Vladimir P Baklaushev, DSc, MD, PhD, Associate Professor, Biomedical Research, Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies, FMBA of Russia, Orekhoviy BLVD, 28, Moscow 115682, Moskva, Russia. serpoff@gmail.com |
| Key Words |
Direct cell reprogramming; Neural precursor cells; Directly reprogrammed neural precursor cells; Spinal cord injury; Nonhuman primates; Regenerative therapy, Evoked potentials |
| Core Tip |
Here, we describe a novel regenerative therapy of spinal cord injury by means of intraspinal transplantation of directly reprogrammed neural precursor cells (drNPCs). Using a somatosensory evoked potential (SSEP)/motor evoked potential (MEP) guided model of spinal cord injury on macaca mulatta we have shown that animals after the transplantation of drNPC demonstrated a significant recovery of the paralyzed hindlimb as well as recovery of SSEP and MEP of injured pathways. Subsequent immunohistochemical analysis showed that drNPCs maintained microtubule associated protein 2 expression characteristic of multipotency in the transplanted spinal cord for at least 12 wk, migrating to areas of axon growth cones. Our data demonstrated that drNPCs transplantation was safe and contributed to improvement of spinal cord function after acute complete spinal cord injury in non-human primates. |
| Publish Date |
2021-05-26 08:55 |
| Citation |
Baklaushev VP, Durov OV, Kalsin VA, Gulaev EV, Kim SV, Gubskiy IL, Revkova VA, Samoilova EM, Melnikov PA, Karal-Ogly DD, Orlov SV, Troitskiy AV, Chekhonin VP, Averyanov AV, Ahlfors JE. Disease modifying treatment of spinal cord injury with directly reprogrammed neural precursor cells in non-human primates. World J Stem Cells 2021; 13(5): 452-469 |
| URL |
https://www.wjgnet.com/1948-0210/full/v13/i5/452.htm |
| DOI |
https://dx.doi.org/10.4252/wjsc.v13.i5.452 |
