Publication Name	World Journal of Methodology
Manuscript ID	6253
Country	Japan

Received	2013-10-11 15:24
Peer-Review Started	2013-10-11 15:41
To Make the First Decision	2013-11-19 13:02
Return for Revision	2013-11-20 18:22
Revised	2013-12-02 19:46
Second Decision	2013-12-13 13:33
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2013-12-13 14:23
Articles in Press
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2013-12-24 10:10
Publish the Manuscript Online	2014-01-14 13:48

ISSN	2222-0682 (online)
Open Access
Copyright
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Website	http://www.wjgnet.com

Category	Methodology
Manuscript Type	Review
Article Title	Sonoporation: Gene transfer using ultrasound
Manuscript Source	Invited Manuscript
All Author List	Minoru Tomizawa, Fuminobu Shinozaki, Yasufumi Motoyoshi, Takao Sugiyama, Shigenori Yamamoto and Makoto Sueishi
Funding Agency and Grant Number
Corresponding Author	Minoru Tomizawa, MD, PhD, Department of Gastroenterology, National Hospital Organization Shimoshizu Hospital, 934-5 Shikawatashi, Yotsukaido City, Chiba 284-0003, Japan. nihminor-cib@umin.ac.jp
Key Words	Gene therapy; Cavity; Microbubbles; Contrast agent; Diagnostic ultrasound
Core Tip	Ultrasound causes cavitation bubbles to form cell membrane pores through which DNA/RNA are transferred. This phenomenon is known as sonoporation. Microbubbles lower the threshold of cavity formation. Sonoporation is less toxic and not associated with tumorigenicity as compared with retroviral and adenoviral vectors. Sonoporation does not require surgical procedure and enhances gene transfer with lipofection. Current limitations of sonoporation are low efficiency of gene transfer and damage of target cells are The use of complexes with chemicals and diagnostic ultrasound are promising approaches to overcome these limitations.
Publish Date	2014-01-14 13:48
Citation	Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S, Sueishi M. Sonoporation: Gene transfer using ultrasound. World J Methodol 2013; 3(4): 39-44
URL	http://www.wjgnet.com/2222-0682/full/v3/i4/39.htm
DOI	http://dx.doi.org/10.4329/wjm.v3.i4.39

Manuscript File	6253-Review.doc
Answering Reviewers	6253-Answering reviewers.pdf
Copyright License Agreement	6253-Copyright assignment.pdf
Non-Native Speakers of English Editing Certificate	6253-Language certificate.pdf
Peer-review Report	6253-Peer review(s).pdf
Scientific Editor Work List	6253-Scientific editor work list.doc