| Category |
Gastroenterology & Hepatology |
| Manuscript Type |
Autobiography |
| Article Title |
Combined probiotic bacteria promotes intestinal epithelial barrier function in interleukin-10-gene-deficient mice
|
| Manuscript Source |
Invited Manuscript |
| All Author List |
Chen-Zhang Shi, Hong-Qi Chen, Yong Liang, Yang Xia, Yong-Zhi Yang, Jun Yang, Jun-Dong Zhang, Shu-Hai Wang, Jing Liu and Huan-Long Qin |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| National Natural Science Foundation Key Projects of China |
81230057 |
| National Natural Science Foundation of China |
81172325 |
| Major Basic Research Program of Shanghai |
12DZ1930502 |
|
| Corresponding Author |
Huan-Long Qin, MD, Professor, Department of Surgery, Affiliated Sixth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University, 600 Yishan Road, Shanghai 200233, China. hlqin10@163.com |
| Key Words |
Probiotic bacteria; Intestinal barrier function; Tight junction proteins; Interleukin-10 gene-deficient mice; Caco-2 monolayers |
| Core Tip |
We investigated the protective effects of combinations of probiotic bacteria (Bifico) on interleukin (IL)-10 gene-deficient (IL-10 KO) mice and Caco-2 cell monolayers. Treatment of IL-10 KO mice with Bifico partially restored body weight, colon length, and epithelial barrier integrity to wild-type levels. Treatment of Caco-2 monolayers with Bifico or single-strain probiotics inhibited enteroinvasive Escherichia coli (EIEC) invasion and reduced secretion of proinflammatory cytokines. Oral administration of Bifico reduced colon inflammation, and directly promoted epithelial barrier function. In addition, Bifico improved epithelial barrier function, and enhanced resistance to EIEC invasion in vitro. |
| Publish Date |
2014-04-29 09:53 |
| Citation |
Shi CZ, Chen HQ, Liang Y, Xia Y, Yang YZ, Yang J, Zhang JD, Wang SH, Liu J, Qin HL. Combined probiotic bacteria promotes intestinal epithelial barrier function in interleukin-10-gene-deficient mice. World J Gastroenterol 2014; 20(16): 4636-4647 |
| URL |
http://www.wjgnet.com/1007-9327/full/v20/i16/4636.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v20.i16.4636 |
