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Articles Published Processes
11/6/2021 4:43:07 AM | Browse: 445 | Download: 938
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Received |
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2021-03-20 12:08 |
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Peer-Review Started |
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2021-03-20 12:11 |
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To Make the First Decision |
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Return for Revision |
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2021-07-03 05:31 |
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Revised |
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2021-07-11 10:52 |
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Second Decision |
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2021-09-18 03:29 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Executive Editor-in-Chief |
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2021-09-19 08:49 |
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Articles in Press |
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2021-09-19 08:49 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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Typeset the Manuscript |
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2021-11-01 01:58 |
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Publish the Manuscript Online |
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2021-11-06 04:43 |
| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non-Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Biochemical Research Methods |
| Manuscript Type |
Minireviews |
| Article Title |
DNA diagnostics for reliable and universal identification of Helicobacter pylori
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| Manuscript Source |
Invited Manuscript |
| All Author List |
Pavol Sulo and Barbora Šipková |
| ORCID |
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| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Slovak Research and Development Agency |
PP-COVID-20-0051 |
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| Corresponding Author |
Pavol Sulo, PhD, Associate Research Scientist, Research Assistant Professor, Research Associate, Department of Biochemistry, Comenius University, Ilkovicova 6, Bratislava 842 15, Slovakia. pavol.sulo@uniba.sk |
| Key Words |
Chronic diseases; Helicobacter pylori; Diagnostics; Nested polymerase chain reaction; DNA sequencing; Detection limit |
| Core Tip |
Polymerase chain reaction (PCR) is not endorsed for Helicobacter pylori (H. pylori) diagnostics due to nonspecific primers and the threat of false-positives. However, a nested PCR that is as specific and equally sensitive in biopsy samples as the 13C-urea breath test was developed. Due to the threshold of H. pylori abundance and the ability to survive in the oral cavity, it is not suitable for saliva samples. Despite DNA degradation in stool samples, nested PCR for a shorter 148 bp amplicon identified twice the number of positive samples as stool antigen test, indicating that many patients are misdiagnosed, not treated by antibiotics, explaining why their problems are interpreted as chronic. |
| Publish Date |
2021-11-06 04:43 |
| Citation |
Sulo P, Šipková B. DNA diagnostics for reliable and universal identification of Helicobacter pylori. World J Gastroenterol 2021; 27(41): 7100-7112 |
| URL |
https://www.wjgnet.com/1007-9327/full/v27/i41/7100.htm |
| DOI |
https://dx.doi.org/10.3748/wjg.v27.i41.7100 |
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