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Articles Published Processes
12/25/2021 8:37:41 AM | Browse: 348 | Download: 764
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Received |
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2021-06-18 14:12 |
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Peer-Review Started |
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2021-06-18 14:13 |
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To Make the First Decision |
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Return for Revision |
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2021-07-17 23:39 |
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Revised |
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2021-08-11 13:06 |
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Second Decision |
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2021-12-09 03:41 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Executive Editor-in-Chief |
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2021-12-10 03:08 |
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Articles in Press |
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2021-12-10 03:08 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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Typeset the Manuscript |
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2021-12-13 09:17 |
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Publish the Manuscript Online |
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2021-12-25 08:37 |
| ISSN |
1948-0210 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Neurosciences |
| Manuscript Type |
Basic Study |
| Article Title |
SmartFlareTM is a reliable method for assessing mRNA expression in single neural stem cells
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| Manuscript Source |
Invited Manuscript |
| All Author List |
Andrea Diana, Maria Dolores Setzu, Zaal Kokaia, Roxana Nat, Cristina Maxia and Daniela Murtas |
| ORCID |
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| Funding Agency and Grant Number |
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| Corresponding Author |
Andrea Diana, PhD, Assistant Professor, Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria di Monserrato SS 554 Bivio per Sestu, Monserrato 09042, Cagliari, Italy. diana@unica.it |
| Key Words |
mRNA detection; SmartFlareTM; NanoFlare; Live staining; Nanotechnology; Neural stem cell genes |
| Core Tip |
The detection of RNA transcripts in living cells is a challenge in embryonic development and cancer related studies. In the last decade, a straightforward and noninvasive approach has emerged, exploiting the combination of nanotechnology and the physiological behavior of stem cells. Although SmartFlareTM technology is far from providing an unambiguous localization of specific mRNAs, it might help in elucidating the time-dependent dynamics of RNA expression at single-cell level, where results are coherent with those coming from both qRT-PCR and fluorescence in situ hybridization (FISH), the gold standards for mRNA analysis. |
| Publish Date |
2021-12-25 08:37 |
| Citation |
Diana A, Setzu MD, Kokaia Z, Nat R, Maxia C, Murtas D. SmartFlareTM is a reliable method for assessing mRNA expression in single neural stem cells. World J Stem Cells 2021; 13(12): 1918-1927 |
| URL |
https://www.wjgnet.com/1948-0210/full/v13/i12/1918.htm |
| DOI |
https://dx.doi.org/10.4252/wjsc.v13.i12.1918 |
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