| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Virology |
| Manuscript Type |
Basic Study |
| Article Title |
Cross-sectional evaluation of circulating hepatitis B virus RNA and DNA: Different quasispecies?
|
| Manuscript Source |
Invited Manuscript |
| All Author List |
Selene Garcia-Garcia, Maria Francesca Cortese, David Tabernero, Josep Gregori, Marta Vila, Beatriz Pacín, Josep Quer, Rosario Casillas, Laura Castillo-Ribelles, Roser Ferrer-Costa, Ariadna Rando-Segura, Jesús Trejo-Zahínos, Tomas Pumarola, Ernesto Casis, Rafael Esteban, Mar Riveiro-Barciela, Maria Buti and Francisco Rodríguez-Frías |
| ORCID |
|
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Instituto de Salud Carlos III |
PI18/01436 |
| European Regional Development Fund (ERDF) |
|
|
| Corresponding Author |
David Tabernero, PhD, Postdoc, Research Scientist, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Instituto de Salud Carlos III, Calle de Melchor Fernández Almagro, 3, Madrid 28029, Spain. david.tabernero@ciberehd.org |
| Key Words |
Hepatitis B virus RNA; Hepatitis B X gene; Quasispecies; Next-generation sequencing; Quasispecies conservation; Quasispecies complexity |
| Core Tip |
Hepatitis B virus (HBV) quasispecies composition and its evolution are related to liver disease progression. HBV-RNA in serum is potentially useful for analyzing viral quasispecies, even in patients with low levels of or suppressed serum HBV-DNA. Few studies have analyzed circulating HBV-RNA quasispecies, and similarities and differences with DNA quasispecies should be assessed. We used next-generation sequencing to analyze RNA quasispecies variability/conservation and complexity, without interference of HBV-DNA, in untreated chronic hepatitis B patients. Comparison of both quasispecies showed similar results between them. DNA quasispecies tended toward greater complexity, while RNA quasispecies tended toward higher variability. |
| Publish Date |
2021-11-06 04:43 |
| Citation |
Garcia-Garcia S, Cortese MF, Tabernero D, Gregori J, Vila M, Pacín B, Quer J, Casillas R, Castillo-Ribelles L, Ferrer-Costa R, Rando-Segura A, Trejo-Zahínos J, Pumarola T, Casis E, Esteban R, Riveiro-Barciela M, Buti M, Rodríguez-Frías F. Cross-sectional evaluation of circulating hepatitis B virus RNA and DNA: Different quasispecies? World J Gastroenterol 2021; 27(41): 7144-7158 |
| URL |
https://www.wjgnet.com/1007-9327/full/v27/i41/7144.htm |
| DOI |
https://dx.doi.org/10.3748/wjg.v27.i41.7144 |
