Publication Name	World Journal of Gastroenterology
Manuscript ID	7023
Country	China

Received	2013-11-02 12:04
Peer-Review Started	2013-11-02 20:27
To Make the First Decision	2014-01-09 18:21
Return for Revision	2014-01-16 22:51
Revised	2014-02-08 11:40
Second Decision	2014-03-05 14:19
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2014-03-05 15:18
Articles in Press
Publication Fee Transferred
Edit the Manuscript by Language Editor	2014-04-11 19:13
Typeset the Manuscript	2014-05-14 15:37
Publish the Manuscript Online	2014-06-06 16:55

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access
Copyright
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Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Cell Biology
Manuscript Type	Observational Study
Article Title	Novel method for extracting exosomes of hepatocellular carcinoma cells
Manuscript Source	Invited Manuscript
All Author List	Lin Zhu, Xiu-Hua Qu, Yu-Lin Sun, Yang-Ming Qian and Xiao-Hang Zhao
Funding Agency and Grant Number	Funding Agency Grant Number State Key Projects for Basic Research 2011CB910703 State Key Projects for Basic Research 2012ZX10002-017 (to Zhao XH) National High-tech R&D Program of China 2013AA041201 (to Qian YM) National High-tech R&D Program of China 2012AA020206 (to Zhao XH) National Natural Science Foundation of China 81372591 National Natural Science Foundation of China 81321091 (to Zhao XH) Research Foundation of the Center for Marine Medicine Rescue of Tsinghua University and NGH to Qian YM Rescue of Tsinghua University and NGH to Zhao XH
Corresponding Author	Xiao-Hang Zhao, Professor, State Key Laboratory of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China. zhaoxh@cicams.ac.cn
Key Words	Exosome; Membrane vesicles; Tissue transglutaminase 2; Annexin A2; Nanomaterials
Core Tip	The traditional extraction assay of exosomes is usually complicated and time consuming. In this manuscript, we investigate a novel assay based on a nanomaterial to extract exosomes more quickly and efficiently compared with the traditional method. A hepatoma-associated protein, tissue transglutaminase 2, was first found to exist in the exosome of the human liver cancer cell line Huh-7. This protein can be secreted through an exosome-mediated non-classical secretion pathway and is a potential tumor marker.
Publish Date	2014-06-06 16:55
Citation	Zhu L, Qu XH, Sun YL, Qian YM, Zhao XH. Novel method for extracting exosomes of hepatocellular carcinoma cells. World J Gastroenterol 2014; 20(21): 6651-6657
URL	http://www.wjgnet.com/1007-9327/full/v20/i21/6651.htm
DOI	http://dx.doi.org/10.3748/wjg.v20.i21.6651

Full Article (PDF)	WJG-20-6651.pdf
Full Article (Word)	WJG-20-6651.doc
Manuscript File	7023-Review.doc
Answering Reviewers	7023-Answering reviewers.pdf
Copyright License Agreement	7023-Copyright assignment.doc
Non-Native Speakers of English Editing Certificate	7023-Language certificate.pdf
Peer-review Report	7023-Peer review(s).pdf
Scientific Editor Work List	7023-Scientific editor work list.doc