Publication Name	World Journal of Gastroenterology
Manuscript ID	73809
Country	China

Received	2021-12-04 08:38
Peer-Review Started	2021-12-04 08:40
To Make the First Decision
Return for Revision	2022-01-08 18:05
Revised	2022-01-14 04:00
Second Decision	2022-05-10 02:52
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2022-05-14 07:43
Articles in Press	2022-05-14 07:43
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2022-06-14 08:34
Publish the Manuscript Online	2022-06-24 13:12

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
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Category	Gastroenterology & Hepatology
Manuscript Type	Clinical and Translational Research
Article Title	Novel multiplex stool-based assay for the detection of early-stage colon cancer in a Chinese population
Manuscript Source	Unsolicited Manuscript
All Author List	Hui-Hong Jiang, Si-Wei Xing, Xuan Tang, Ying Chen, Kang Lin, Lu-Wei He, Mou-Bin Lin and Er-Jiang Tang
ORCID	Author(s) ORCID Number Hui-Hong Jiang http://orcid.org/0000-0002-7391-1767 Si-Wei Xing http://orcid.org/0000-0002-7222-1043 Xuan Tang http://orcid.org/0000-0002-5895-3467 Ying Chen http://orcid.org/0000-0001-5084-7507 Kang Lin http://orcid.org/0000-0002-3485-6574 Lu-Wei He http://orcid.org/0000-0001-6564-848X Mou-Bin Lin http://orcid.org/0000-0002-0686-688X Er-Jiang Tang http://orcid.org/0000-0002-8042-5994
Funding Agency and Grant Number	Funding Agency Grant Number Shanghai Pujiang Program 21PJD066 Shanghai Municipal Commission of Health and Family Planning ZK2019A19 Shanghai Municipal Science and Technology Commission 19411971500 Shanghai Yangpu District Science and Technology Commission YPM202101
Corresponding Author	Er-Jiang Tang, MSc, Statistician, Center for Clinical Research and Translational Medicine, Yangpu Hospital, Tongji University, No. 450 Tengyue Road, Shanghai 200090, China. tangerjiang1988051@163.com
Key Words	Colon cancer; Early screening; Stool biomarker; DNA methylation; Fecal immunochemical test
Core Tip	Stool DNA (sDNA) methylation analysis has a promising application in the early diagnosis of colorectal cancer. However, reliable biomarkers for detecting early-stage colon cancer (ECC) are lacking. In this study, by targeted bisulfite sequencing, we identified a novel multiplex stool-based assay combining three sDNA methylation biomarkers and fecal immunochemical test. Further validation in larger samples by pyrosequencing showed that it enabled the diagnosis of ECC with high sensitivity and specificity throughout the colon. To our knowledge, this is the first study to focus on ECC screening in China.
Publish Date	2022-06-24 13:12
Citation	Jiang HH, Xing SW, Tang X, Chen Y, Lin K, He LW, Lin MB, Tang EJ. Novel multiplex stool-based assay for the detection of early-stage colon cancer in a Chinese population. World J Gastroenterol 2022; 28(24): 2705-2720
URL	https://www.wjgnet.com/1007-9327/full/v28/i24/2705.htm
DOI	https://dx.doi.org/10.3748/wjg.v28.i24.2705

Full Article (PDF)	WJG-28-2705.pdf
Full Article (Word)	WJG-28-2705.docx
Manuscript File	73809_Auto_Edited-LS.docx
Answering Reviewers	73809-Answering reviewers.pdf
Audio Core Tip	73809-Audio core tip.MP3
Biostatistics Review Certificate	73809-Biostatistics statement.PDF
Clinical Trial Registration Statement	73809-Clinical trial registration statement.PDF
Conflict-of-Interest Disclosure Form	73809-Conflict-of-interest statement.pdf
Copyright License Agreement	73809-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	73809-Grant application form(s).pdf
Signed Informed Consent Form(s) or Document(s)	73809-Informed consent statement.PDF
Institutional Review Board Approval Form or Document	73809-Institutional review board statement.PDF
Non-Native Speakers of English Editing Certificate	73809-Language certificate.pdf
Supplementary Material	73809-Supplementary material.pdf
Peer-review Report	73809-Peer-review(s).pdf
Scientific Misconduct Check	73809-Bing-Wang JJ-2.png
Scientific Editor Work List	73809-Scientific editor work list.pdf
CrossCheck Report	73809-CrossCheck report.pdf