Publication Name	World Journal of Stem Cells
Manuscript ID	73978
Country	China

Received	2021-12-10 14:44
Peer-Review Started	2021-12-10 14:46
To Make the First Decision
Return for Revision	2022-03-13 22:51
Revised	2022-04-07 17:43
Second Decision	2022-05-23 03:30
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2022-05-28 07:22
Articles in Press	2022-05-28 07:22
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2022-06-21 00:49
Publish the Manuscript Online	2022-06-24 10:26

ISSN	1948-0210 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
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Category	Neurosciences
Manuscript Type	Review
Article Title	Application and prospects of high-throughput screening for in vitro neurogenesis
Manuscript Source	Invited Manuscript
All Author List	Shu-Yuan Zhang, Juan Zhao, Jun-Jun Ni, Hui Li, Zhen-Zhen Quan and Hong Qing
ORCID	Author(s) ORCID Number Shu-Yuan Zhang http://orcid.org/0000-0001-6925-2140 Juan Zhao http://orcid.org/0000-0002-9719-751X Jun-Jun Ni http://orcid.org/0000-0001-8486-8146 Hui Li http://orcid.org/0000-0002-7392-0233 Zhen-Zhen Quan http://orcid.org/0000-0002-2557-0371 Hong Qing http://orcid.org/0000-0003-2820-450X
Funding Agency and Grant Number	Funding Agency Grant Number National Natural Science Foundation of China 81870844 National Natural Science Foundation of China 82001167 National Natural Science Foundation of China 82101394
Corresponding Author	Hong Qing, MD, PhD, Professor, Key Laboratory of Molecular Medicine and Biotherapy in the Ministry of Industry and Information Technology, Department of Biology, School of Life Science, Beijing Institute of Technology, No. 5 Yard, Zhong Guan Cun South Street, Haidian District, Beijing 100081, China. hqing@bit.edu.cn
Key Words	High-throughput screening; Stem cells; Neurogenesis; Cell differentiation; Three-dimensional cell culture; Cellular microenvironments
Core Tip	High-throughput screening (HTS) is a promising technology that can screen out targets from thousands of candidates. Here, we review the evidence that HTS could be beneficial in neurogenesis methods in various ways: The HTS method can screen out specific genes that induce neural induction, small molecules that facilitate neural differentiation, and three-dimensional microenvironments that could better modulate the microenvironments in vivo. We also focus on the application and prospects of HTS in in vitro neurogenesis, as organoid-based and microfluidic platforms are needed for future research.
Publish Date	2022-06-24 10:26
Citation	Zhang SY, Zhao J, Ni JJ, Li H, Quan ZZ, Qing H. Application and prospects of high-throughput screening for in vitro neurogenesis. World J Stem Cells 2022; 14(6): 393-491
URL	https://www.wjgnet.com/1948-0210/full/v14/i6/393.htm
DOI	https://dx.doi.org/10.4252/wjsc.v14.i6.393

Full Article (PDF)	WJSC-14-393.pdf
Full Article (Word)	WJSC-14-393.docx
Manuscript File	73978_Auto_Edited-LS.docx
Answering Reviewers	73978-Answering reviewers.pdf
Audio Core Tip	73978-Audio core tip.m4a
Conflict-of-Interest Disclosure Form	73978-Conflict-of-interest statement.pdf
Copyright License Agreement	73978-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	73978-Grant application form(s).pdf
Non-Native Speakers of English Editing Certificate	73978-Language certificate.pdf
Supplementary Material	73978-Supplementary material.pdf
Peer-review Report	73978-Peer-review(s).pdf
Scientific Misconduct Check	73978-Bing-Fan JR-2.png
Scientific Editor Work List	73978-Scientific editor work list.pdf
CrossCheck Report	73978-CrossCheck report.pdf
CrossCheck Report	73978-CrossCheck.png