Publication Name	World Journal of Biological Chemistry
Manuscript ID	7552
Country	Venezuela

Received	2013-11-24 12:08
Peer-Review Started	2013-11-24 14:02
To Make the First Decision	2013-12-25 18:11
Return for Revision	2013-12-26 20:25
Revised	2014-02-10 12:32
Second Decision	2014-04-17 14:46
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2014-04-17 15:07
Articles in Press
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2014-05-22 14:39
Publish the Manuscript Online	2014-06-11 10:30

ISSN	1949-8454 (online)
Open Access
Copyright
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Category	Biochemistry & Molecular Biology
Manuscript Type	Autobiography
Article Title	Binding of rhodopsin and rhodopsin analogues to transducin, rhodopsin kinase and arrestin-1
Manuscript Source	Invited Manuscript
All Author List	Nelson A Araujo, Carlos E Sanz-Rodríguez and José Bubis
Funding Agency and Grant Number	Funding Agency Grant Number FONACIT, Caracas, Venezuela S1-2000000514 FONACIT, Caracas, Venezuela LAB-2000001639 Decanato de Investigación y Desarrollo, Universidad Simón Bolívar, Caracas, Venezuela S1-IN-CB-001-09
Corresponding Author	José Bubis, PhD, Departamento de Biología Celular, Universidad Simón Bolívar, Apartado 89000, Valle de Sartenejas, Baruta, Caracas 1081-A, Venezuela. jbubis@usb.ve
Key Words	Rhodopsin; Rhodopsin analogues; 9-cis-Retinal; 11-cis-Retinal; 13-cis-Retinal; Photointermediates; Transducin; Rhodopsin kinase; Arrestin-1; Visual process
Core Tip	Rhodopsin is a specialized G protein-coupled receptors composed of a single polypeptide chain, opsin, and a covalently linked 11-cis-retinal. It is well known that rhodopsin uses the 11-cis form of retinal exclusively as the chromophore. Retinal analogues have long been used to probe the chromophore binding pocket and to study ligand-protein relationships to better understand the photochemical cis-trans isomerization of rhodopsin. However, little is known about the interactions of rhodopsin analogues with other proteins in the visual cascade. Here, we were able to reconstitute a rhodopsin analogue containing 13-cis-retinal. We compared the binding of reconstituted rhodopsin, 9-cis-retinal-rhodopsin and 13-cis-retinal-rhodopsin to transducin, rhodopsin kinase and arrestin-1, both in the dark and under illumination. Interestingly, we found that in the dark the rhodopsin analogue containing the 13-cis isomer of retinal appears to fold in a pseudo-active conformation that mimics the active photointermediate of rhodopsin.
Publish Date	2014-06-11 10:30
Citation	Araujo NA, Sanz-Rodríguez CE, Bubis J. Binding of rhodopsin and rhodopsin analogues to transducin, rhodopsin kinase and arrestin-1. World J Biol Chem 2014; 5(2): 254-268
URL	http://www.wjgnet.com/1949-8454/full/v5/i2/254.htm
DOI	http://dx.doi.org/10.4331/wjbc.v5.i2.254

Full Article (PDF)	wjbc-5-254.pdf
Full Article (Word)	wjbc-5-254.doc
Manuscript File	7552-Review.docx
Answering Reviewers	7552-Answering reviewers.pdf
Copyright License Agreement	7552-Copyright assignment.pdf
Non-Native Speakers of English Editing Certificate	7552-Language certificate.pdf
Peer-review Report	7552-Peer review.pdf
Scientific Editor Work List	7552-Scientific editor work list.doc