Publication Name	World Journal of Stem Cells
Manuscript ID	78888
Country	China

Received	2022-07-20 09:19
Peer-Review Started	2022-07-20 09:22
To Make the First Decision
Return for Revision	2022-08-08 02:19
Revised	2022-10-05 09:55
Second Decision	2022-11-17 04:28
Accepted by Journal Editor-in-Chief	2022-11-18 02:11
Accepted by Executive Editor-in-Chief	2022-11-23 00:03
Articles in Press	2022-11-23 00:03
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2022-11-24 10:31
Publish the Manuscript Online	2022-11-25 11:36

ISSN	1948-0210 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
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Category	Cell Biology
Manuscript Type	Basic Study
Article Title	Tumor necrosis factor-α inhibition restores matrix formation by human adipose-derived stem cells in the late stage of chondrogenic differentiation
Manuscript Source	Unsolicited Manuscript
All Author List	Jiang-Tao Wan, Xian-Shuai Qiu, Zhuo-Hang Fu, Yong-Can Huang and Shao-Xiong Min
ORCID	Author(s) ORCID Number Yong-Can Huang http://orcid.org/0000-0001-8548-8233 Shao-Xiong Min http://orcid.org/0000-0003-3334-6254
Funding Agency and Grant Number	Funding Agency Grant Number Natural Science Foundation of Guangdong Province, China 2017A030313564 Shenzhen High-level Hospital Construction Fund to Peking University Shenzhen Hospital
Corresponding Author	Shao-Xiong Min, PhD, Chief Physician, Department of Spine Surgery, Peking University Shenzhen Hospital, No. 1120 Lianhua Road, Futian District, Shenzhen 518036, Guangdong Province, China. msxbear24@163.com
Key Words	Adipose-derived mesenchymal stem cells; Human adipose-derived mesenchymal stem cells; Chondrogenic differentiation; Tumor necrosis factor-alpha; Etanercept; Infliximab; Nuclear factor kappa-B
Core Tip	Adipose stem cells are important seed cells that are used in cartilage tissue engineering. However, at present, cartilage matrix secretion by adipose-derived stem cells (ADSCs) inevitably decreases during the late stage of induced chondrogenic differentiation in vitro, which seriously limits the further application of ADSCs. Our team found that the level of inflammation in the culture system, mainly the levels of tumor necrosis factor-alpha (TNF-α) and matrix metalloproteinase 3, continuously increased during the chondrogenic differentiation of ADSCs. To address this issue, our team added etanercept or infliximab, which are targeted inhibitors of TNF-α, to the chondrogenic differentiation induction medium and successfully restored matrix formation by human ADSCs in the late stage of chondrogenic differentiation. Further studies found that these effects were achieved by reducing NF-κB pathway activation.
Publish Date	2022-11-25 11:36
Citation	Wan JT, Qiu XS, Fu ZH, Huang YC, Min SX. Tumor necrosis factor-α inhibition restores matrix formation by human adipose-derived stem cells in the late stage of chondrogenic differentiation. World J Stem Cells 2022; 14(11): 798-814
URL	https://www.wjgnet.com/1948-0210/full/v14/i11/798.htm
DOI	https://dx.doi.org/10.4252/wjsc.v14.i11.798

Full Article (PDF)	WJSC-14-798.pdf
Full Article (Word)	WJSC-14-798.docx
Manuscript File	78888_Auto_Edited-LM.docx
Answering Reviewers	78888-Answering reviewers.pdf
Audio Core Tip	78888-Audio core tip.wav
Conflict-of-Interest Disclosure Form	78888-Conflict-of-interest statement.pdf
Copyright License Agreement	78888-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	78888-Grant application form(s).pdf
Institutional Review Board Approval Form or Document	78888-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate	78888-Language certificate.pdf
Supplementary Material	78888-Supplementary material.pdf
Peer-review Report	78888-Peer-review(s).pdf
Scientific Misconduct Check	78888-CrossCheck.png
Scientific Editor Work List	78888-Scientific editor work list.pdf
CrossCheck Report	78888-CrossCheck report.pdf