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Articles Published Processes
7/25/2023 6:43:29 AM | Browse: 207 | Download: 561
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Received |
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2023-03-21 15:00 |
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Peer-Review Started |
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2023-03-21 15:03 |
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To Make the First Decision |
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Return for Revision |
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2023-05-22 17:18 |
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Revised |
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2023-06-01 09:36 |
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Second Decision |
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2023-06-21 02:58 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Executive Editor-in-Chief |
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2023-06-21 08:56 |
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Articles in Press |
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2023-06-21 08:56 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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Typeset the Manuscript |
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2023-07-20 03:04 |
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Publish the Manuscript Online |
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2023-07-25 06:43 |
| ISSN |
1948-0210 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Cell Biology |
| Manuscript Type |
Basic Study |
| Article Title |
Generation of a human haploid neural stem cell line for genome-wide genetic screening
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| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Hai-Song Wang, Xin-Rui Ma, Wen-Bin Niu, Hao Shi, Yi-Dong Liu, Ning-Zhao Ma, Nan Zhang, Zi-Wei Jiang and Ying-Pu Sun |
| ORCID |
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| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| National Natural Science Foundation of China |
81901476 |
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| Corresponding Author |
Hai-Song Wang, Doctor, PhD, Assistant Professor, Center for Reproductive Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, No. 40 Daxue Road, Zhengzhou 450052, Henan Province, China. hswang813@zzu.edu.cn |
| Key Words |
Haploid; Neural stem cells; Extended pluripotency; Genetic screening; Cell therapy |
| Core Tip |
Human embryonic stem cells are widely used in preclinical and genetic screening studies. Haploid cells are ideal tools to perform genetic screening. To date, no human haploid somatic cell lines have been successfully created. We converted human haploid embryonic stem cells to an extended pluripotency state by optimizing the culture medium. The derived haploid neural stem cells can proliferate as a haploid genome and maintain multipotency to generate functional neurons and glia. The haploid neural stem cells can also be easily used for gene editing to generate numerous homozygous mutations for lineage-specific screens. |
| Publish Date |
2023-07-25 06:43 |
| Citation |
Wang HS, Ma XR, Niu WB, Shi H, Liu YD, Ma NZ, Zhang N, Jiang ZW, Sun YP. Generation of a human haploid neural stem cell line for genome-wide genetic screening. World J Stem Cells 2023; 15(7): 734-750 |
| URL |
https://www.wjgnet.com/1948-0210/full/v15/i7/734.htm |
| DOI |
https://dx.doi.org/10.4252/wjsc.v15.i7.734 |
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