| Category |
Oncology |
| Manuscript Type |
Autobiography |
| Article Title |
Identification of biomarkers for hepatocellular carcinoma by semiquantitative immunocytochemistry
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Hong Mu, Kai-Xuan Lin, Hong Zhao, Shu Xing, Cong Li, Fang Liu, Hai-Zhen Lu, Ze Zhang, Yu-Lin Sun, Xi-Yun Yan, Jian-Qiang Cai and Xiao-Hang Zhao |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| National High-tech R&D Program of China |
2012AA020206 |
| Key Project for the Infectious Diseases |
2012ZX10002-017 |
| Key Project for the Infectious Diseases |
2013ZX10002009-001-004 |
| State Key Projects for Basic Research |
2011CB910703 |
| National Natural Science Foundation of China |
81372591 |
| National Natural Science Foundation of China |
81321091 |
| Center for Marine Medicine and Rescue of Tsinghua University |
|
|
| Corresponding Author |
Xiao-Hang Zhao, MD, PhD, State Key Laboratory of Molecular Oncology, Cancer Institute Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Panjiayuan Nanli 17, Chaoyang District, Beijing 100021, China. zhaoxh@cicams.ac.cn |
| Key Words |
Hepatocellular carcinoma; Biomarker; Immunocytochemistry; Semiquantitative analysis; Three-dimensional reconstruction |
| Core Tip |
We report a novel workflow to detect potentially valuable biomarkers for hepatocellular carcinoma (HCC). We measured immunofluorescence intensity and performed statistical analyses to assess the expression of biomarkers in cell lines and patient blood samples. Furthermore, we determined the expression of biomarkers via three-dimensional reconstructions. These analyses indicated that asialoglycoprotein receptor (ASGPR), glypican-3 (GPC3), and cytokeratin (CK) may be valuable HCC biomarkers for detecting circulating tumor cells (CTCs). In addition, the expression of ASGPR and GPC3 might correlate with patients’ prognoses, and our CTC detection method can include epithelial cell adhesion molecule- and vimentin-positive tumor cells, and will thus supplement previous studies and potentially help predict future tumor recurrence and metastasis. |
| Publish Date |
2014-05-18 12:31 |
| Citation |
Mu H, Lin KX, Zhao H, Xing S, Li C, Liu F, Lu HZ, Zhang Z, Sun YL, Yan XY, Cai JQ, Zhao XH. Identification of biomarkers for hepatocellular carcinoma by semiquantitative immunocytochemistry. World J Gastroenterol 2014; 20(19): 5826-5838 |
| URL |
http://www.wjgnet.com/1007-9327/full/v20/i19/5826.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v20.i19.5826 |
