Publication Name	World Journal of Gastroenterology
Manuscript ID	8697
Country	China

Received	2014-01-03 15:11
Peer-Review Started	2014-01-03 19:44
To Make the First Decision	2014-02-14 17:49
Return for Revision	2014-02-18 21:10
Revised	2014-03-18 13:03
Second Decision	2014-04-29 12:56
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2014-04-29 14:10
Articles in Press	2014-05-23 09:27
Publication Fee Transferred
Edit the Manuscript by Language Editor	2014-05-17 05:25
Typeset the Manuscript	2014-08-19 22:03
Publish the Manuscript Online	2014-09-07 13:31

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access
Copyright
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Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Oncology
Manuscript Type	Retrospective Study
Article Title	Identification of differential proteins in colorectal cancer cells treated with caffeic acid phenethyl ester
Manuscript Source	Unsolicited Manuscript
All Author List	Yu-Jun He, Wan-Ling Li, Bao-Hua Liu, Hui Dong, Zhi-Rong Mou and Yu-Zhang Wu
Funding Agency and Grant Number	Funding Agency Grant Number National Natural Science Foundation of China 30872466 National Natural Science Foundation of China 30801096 Natural Science Foundation of Chongqing 2011BB5032 PLA Logistics Science Research during the 12th Five-Year Plan Period BWS11J041
Corresponding Author	Zhi-Rong Mou, PhD, Institute of Immunology of PLA, the Third Military Medical University, Chongqing 400038, China. mouzr@yahoo.com
Key Words	Caffeic acid phenethyl ester; Colorectal cancer; Proteomics; Two-dimensional electrophoresis; Mass spectrometry
Core Tip	To investigate the molecular mechanisms of the anti-cancer activity of caffeic acid phenethyl ester (CAPE), CAPE-treated colorectal cancer SW480 cells were analysed by a 2D-gel based proteomics approach. Four up-regulated proteins and seven down-regulated proteins in CAPE-treated SW480 cells were found and further identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry following a database search. The down-regulated proteins, PSMA1 and PSAT1 and up-regulated proteins GNPDA1 and GPX-1 were validated by Western blotting. The two tumorigenesis associated proteins, PSMA1 and PSAT1, were further confirmed by immunofluorescence assay. These differentiated proteins in colorectal cancer cells following CAPE treatment, may be potential molecular targets of CAPE and involved in the anti-cancer effect of CAPE.
Publish Date	2014-09-07 13:31
Citation	He YJ, Li WL, Liu BH, Dong H, Mou ZR, Wu YZ. Identification of differential proteins in colorectal cancer cells treated with caffeic acid phenethyl ester. World J Gastroenterol 2014; 20(33): 11840-11849
URL	http://www.wjgnet.com/1007-9327/full/v20/i33/11840.htm
DOI	http://dx.doi.org/10.3748/wjg.v20.i33.11840

Full Article (PDF)	WJG-20-11840.pdf
Full Article (Word)	WJG-20-11840.doc
Manuscript File	8697-Review.doc
Answering Reviewers	8697-Answering reviewers.pdf
Copyright License Agreement	8697-Copyright assignment.PDF
Non-Native Speakers of English Editing Certificate	8697-Language certificate.pdf
Peer-review Report	8697-Peer review.pdf
Scientific Editor Work List	8697-Scientific editor work list.pdf