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Articles Published Processes
12/20/2023 2:52:02 PM | Browse: 130 | Download: 433
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Received |
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2023-07-21 17:40 |
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Peer-Review Started |
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2023-07-21 17:42 |
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To Make the First Decision |
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Return for Revision |
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2023-08-31 07:22 |
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Revised |
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2023-09-07 19:22 |
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Second Decision |
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2023-10-17 03:05 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Executive Editor-in-Chief |
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2023-10-23 02:32 |
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Articles in Press |
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2023-10-23 02:32 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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Typeset the Manuscript |
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2023-12-13 07:53 |
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Publish the Manuscript Online |
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2023-12-20 14:52 |
| ISSN |
2222-0682 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Transplantation |
| Manuscript Type |
Basic Study |
| Article Title |
Urine exosome mRNA-based test for monitoring kidney allograft rejection: Effects of sample transportation and storage, and interference substances
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| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Matt McFaul, Chris Ventura, Sean Evans, Halil Dundar, Marc J Rumpler, Christopher McCloskey, Dave Lowe and Alexandre V Vlassov |
| ORCID |
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| Funding Agency and Grant Number |
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| Corresponding Author |
Alexandre V Vlassov, PhD, Director, Director, Department of Research and Development, Thermo Fisher Scientific, 22801 Roscoe Blvd, West Hills, CA 91304, United States. sasha.vlassov@thermofisher.com |
| Key Words |
Kidney allograft; Post-transplant monitoring; Liquid biopsy; Exosome; mRNA; Quantitative polymerase chain reaction |
| Core Tip |
Recently, it was demonstrated that analysis of urine-derived exosomal mRNA allows early detection of kidney allograft rejection. We further studied exosomes and their mRNA content as a highly promising diagnostic modality. This included stability studies of urine samples and exo-mRNA upon transportation from the point of collection to centralized testing facility, short-term storage of urine at different conditions upon receipt till the point molecular assay is performed, and effects of various interfering substances on the quantitative polymerase chain reaction assay. mRNA from the urine-derived exosomes was proven to be stable across broad range of conditions and produce robust results in post-transplant monitoring assays. |
| Publish Date |
2023-12-20 14:52 |
| Citation |
McFaul M, Ventura C, Evans S, Dundar H, Rumpler MJ, McCloskey C, Lowe D, Vlassov AV. Urine Exosome mRNA-based test for monitoring kidney allograft rejection: Effects of sample transportation and storage, and interference substances. World J Methodol 2023; 13(5): 492-501 |
| URL |
https://www.wjgnet.com/2222-0682/full/v13/i5/492.htm |
| DOI |
https://dx.doi.org/10.5662/wjm.v13.i5.492 |
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