| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Gastroenterology & Hepatology |
| Manuscript Type |
Basic Study |
| Article Title |
First report on establishment and characterization of the extrahepatic cholangiocarcinoma sarcoma cell line CBC2T-2
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Ning-Zu Jiang, Ming-Zhen Bai, Chong-Fei Huang, Ze-Long Ma, Ru-Yang Zhong, Wen-Kang Fu, Long Gao, Liang Tian, Ning-Ning Mi, Hai-Dong Ma, Ya-Wen Lu, Zi-Ang Zhang, Jin-Yu Zhao, Hai-Ying Yu, Bao-Ping Zhang, Xian-Zhuo Zhang, Yan-Xian Ren, Chao Zhang, Yong Zhang, Ping Yue, Yan-Yan Lin and Wen-Bo Meng |
| ORCID |
|
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| National Natural Science Foundation of China |
82060551 |
| Lanzhou Chengguan District Science and Technology Planning Project |
2019JSCX0092 |
|
| Corresponding Author |
Wen-Bo Meng, MD, PhD, Doctor, Doctor, Professor, Department of General Surgery, The First Hospital of Lanzhou University and Laboratory of Biological Therapy and Regenerative Medicine Transformation Gansu Province, No. 1 Donggang West Road, Chengguan District, Lanzhou 730000, Gansu Province, China. mengwb@lzu.edu.cn |
| Key Words |
Carcinosarcoma; Drug resistance; Xenograft; Cell line; Establishment |
| Core Tip |
The study established and characterized the CBC2T-2 cell line as a potential model for studying human extrahepatic cholangiocarcinoma sarcomas. The cells exhibited both epithelial and mesenchymal characteristics and demonstrated high proliferation, migration, invasion, and clonogenic abilities. Chromosomal analysis revealed polyploidy with varying chromosome numbers and in vivo tumorigenicity was confirmed in non-obese diabetic/severe combined immunodeficient mice. Immunohistochemistry indicated positive expression for both epithelial marker CK19 and mesenchymal marker vimentin. The cell line was also used to screen clinical agents, and paclitaxel and gemcitabine exhibited optimal effects. Whole-exome sequencing further revealed genetic insights. |
| Publish Date |
2023-11-07 06:50 |
| Citation |
Jiang NZ, Bai MZ, Huang CF, Ma ZL, Zhong RY, Fu WK, Gao L, Tian L, Mi NN, Ma HD, Lu YW, Zhang ZA, Zhao JY, Yu HY, Zhang BP, Zhang XZ, Ren YX, Zhang C, Zhang Y, Yue P, Lin YY, Meng WB. First report on establishment and characterization of the extrahepatic cholangiocarcinoma sarcoma cell line CBC2T-2. World J Gastroenterol 2023; 29(41): 5683-5698 |
| URL |
https://www.wjgnet.com/1007-9327/full/v29/i41/5683.htm |
| DOI |
https://dx.doi.org/10.3748/wjg.v29.i41.5683 |
