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Articles Published Processes
6/25/2024 10:11:57 AM | Browse: 92 | Download: 272
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Received |
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2023-11-23 12:57 |
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Peer-Review Started |
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2023-11-23 12:57 |
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To Make the First Decision |
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Return for Revision |
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2023-12-21 21:52 |
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Revised |
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2024-01-23 18:28 |
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Second Decision |
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2024-04-01 01:40 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Executive Editor-in-Chief |
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2024-04-12 06:53 |
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Articles in Press |
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2024-04-12 06:53 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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2024-04-19 03:32 |
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Typeset the Manuscript |
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2024-05-23 11:55 |
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Publish the Manuscript Online |
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2024-06-25 10:11 |
| ISSN |
1948-0210 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Biochemistry & Molecular Biology |
| Manuscript Type |
Basic Study |
| Article Title |
RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation
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| Manuscript Source |
Invited Manuscript |
| All Author List |
Daniel B Ferreira, Leticia M Gasparoni, Cristiane F Bronzeri and Katiucia BS Paiva |
| ORCID |
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| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| São Paulo Research Foundation (FAPESP) |
2010/08918-9, 2020/11564-6 |
| KBSP Young Investigator Fellowship |
2011/00204-0 |
| DBF Fellowship |
2019/27492-7 |
| LMG Fellowship |
2014/01395-1 |
| CFB Fellowship |
2014/14278-3 |
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| Corresponding Author |
Katiucia BS Paiva, BSc, PhD, Assistant Professor, Laboratory of Extracellular Matrix Biology and Cellular Interaction, Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes n. 2.415 (ICB3 - laboratório 111), São Paulo 05508-000, São Paulo, Brazil. katipaiva@usp.br |
| Key Words |
Dental pulp stem cells; Reference gene; Housekeeping gene; Endogenous gene; Osteogenic differentiation; RefFinder |
| Core Tip |
Detecting the best reference genes (RG) under specific conditions has been recommended as a good practice to improve the understanding of gene expression. Stem cells have been largely studied during commitment to particular cell lineages for many applications, such as tissue engineering. In this way, dental pulp stem cells (DPSCs) are a promise for craniofacial reconstruction. For the first time, we show that the best pair of RGs for osteogenic differentiation of human DPSCs are (ribosomal protein, large, P0)/(TATA box binding protein) by quantitative real-time polymerase chain reaction through the four algorithms (ΔCt comparative method, GeNorm, BestKeeper, and NormFinder) and ranked by RefFinder. |
| Publish Date |
2024-06-25 10:11 |
| Citation |
<p>Ferreira DB, Gasparoni LM, Bronzeri CF, Paiva KB. RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation. <i>World J Stem Cells</i> 2024; 16(6): 656-669</p> |
| URL |
https://www.wjgnet.com/1948-0210/full/v16/i6/656.htm |
| DOI |
https://dx.doi.org/10.4252/wjsc.v16.i6.656 |
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