Publication Name	World Journal of Methodology
Manuscript ID	89723
Country	Brazil

Received	2023-11-10 13:01
Peer-Review Started	2023-11-10 13:02
To Make the First Decision
Return for Revision	2023-12-18 12:08
Revised	2023-12-26 16:24
Second Decision	2024-02-18 01:54
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2024-02-18 06:19
Articles in Press	2024-02-18 06:19
Publication Fee Transferred
Edit the Manuscript by Language Editor	2024-02-13 04:12
Typeset the Manuscript	2024-02-28 01:38
Publish the Manuscript Online	2024-03-07 09:32

ISSN	2222-0682 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
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Category	Biochemistry & Molecular Biology
Manuscript Type	Basic Study
Article Title	Time-dependent impact of a high-fat diet on the intestinal barrier of male mice
Manuscript Source	Invited Manuscript
All Author List	Carolline Santos Miranda, Daiana Araujo Santana-Oliveira, Isabela Lopes Vasques-Monteiro, Nathan Soares Dantas-Miranda, Jade Sancha de Oliveira Glauser, Flavia Maria Silva-Veiga and Vanessa Souza-Mello
ORCID	Author(s) ORCID Number Carolline Santos Miranda http://orcid.org/0000-0001-5488-0179 Daiana Araujo Santana-Oliveira http://orcid.org/0000-0002-2474-5846 Isabela Lopes Vasques-Monteiro http://orcid.org/0000-0003-1729-3201 Nathan Soares Dantas-Miranda http://orcid.org/0009-0000-8507-3062 Jade Sancha de Oliveira Glauser http://orcid.org/0009-0009-9065-967X Flavia Maria Silva-Veiga http://orcid.org/0000-0002-3320-5825 Vanessa Souza-Mello http://orcid.org/0000-0002-2510-9569
Funding Agency and Grant Number
Corresponding Author	Vanessa Souza-Mello, PhD, Associate Professor, Department of Anatomy, Rio de Janeiro State University, Blvd. 28 de setembro 87, fundos, Rio de Janeiro 20551030, Brazil. souzamello.uerj@gmail.com
Key Words	High-fat diet; Intestine; Ultrastructure; Goblet cells; Gut microbiota
Core Tip	There is great interest in the scientific community in the impact of unhealthy eating habits, such as excess saturated fatty acid intake, on the gut microbiota composition and metabolic disease onset. Here, we evaluated the progressive changes in the intestinal structural barrier and gut microbiota composition in mice fed a high-fat diet (HFD) for 10 or 16 wk. HFD administration resulted in gut dysbiosis, compensatory enhancement of goblet cell numerical density, and increased Mucin2 expression after 10 wk. Continuous feeding reduced the goblet cell number and the expression of Mucin2 and occludin, consistent with the impaired tight junction ultrastructure in the chronically obese HFD-fed mice after 16 wk.
Publish Date	2024-03-07 09:32
Citation	Miranda CS, Santana-Oliveira DA, Vasques-Monteiro IL, Dantas-Miranda NS, Glauser JSO, Silva-Veiga FM, Souza-Mello V. Time-dependent impact of a high-fat diet on the intestinal barrier of male mice. World J Methodol 2024; 14(1): 89723
URL	https://www.wjgnet.com/2222-0682/full/v14/i1/89723.htm
DOI	https://dx.doi.org/10.5662/wjm.v14.i1.89723

Full Article (PDF)	WJM-14-89723-with-cover.pdf
The ARRIVE Guidelines	89723-The ARRIVE guidelines.pdf
Manuscript File	89723_Auto_Edited-JJW-WangTQ-YJP.docx
Answering Reviewers	89723-Answering reviewers.pdf
Audio Core Tip	89723-Audio core tip.mp3
Biostatistics Review Certificate	89723-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	89723-Conflict-of-interest statement.pdf
Copyright License Agreement	89723-Copyright license agreement.pdf
Institutional Animal Care and Use Committee Approval Form or Document	89723-Institutional animal care and use committee statement.pdf
Non-Native Speakers of English Editing Certificate	89723-Language certificate.pdf
Peer-review Report	89723-Peer-review(s).pdf
Scientific Misconduct Check	89723-Bing-Wang JJ-2.png
Scientific Editor Work List	89723-Scientific editor work list.pdf