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Articles Published Processes
6/15/2024 2:49:03 AM | Browse: 87 | Download: 601
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Received |
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2023-12-26 07:37 |
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Peer-Review Started |
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2023-12-26 07:37 |
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First Decision by Editorial Office Director |
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2024-01-27 05:55 |
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Return for Revision |
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2024-01-28 16:30 |
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Revised |
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2024-02-18 19:55 |
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Publication Fee Transferred |
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Second Decision by Editor |
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2024-04-07 01:10 |
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Second Decision by Editor-in-Chief |
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Final Decision by Editorial Office Director |
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2024-04-07 01:45 |
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Articles in Press |
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2024-04-07 01:45 |
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Edit the Manuscript by Language Editor |
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Typeset the Manuscript |
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2024-04-29 01:39 |
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Publish the Manuscript Online |
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2024-06-15 02:49 |
| ISSN |
1948-5204 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Cell Biology |
| Manuscript Type |
Basic Study |
| Article Title |
Fine-needle aspiration technique under endoscopic ultrasound guidance: A technical approach for RNA profiling of pancreatic neoplasms
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| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Sabina Sherafedinovna Seyfedinova, Olga Aleksandrovna Freylikhman, Polina Sergeevna Sokolnikova, Konstantin Aleksandrovich Samochernykh, Anna Aleksandrovna Kostareva, Olga Viktorovna Kalinina and Evgeniy Gennadievich Solonitsyn |
| ORCID |
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| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Ministry of Science and Higher Education of the Russian Federation |
075-15-2022-301 |
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| Corresponding Author |
Sabina Sherafedinovna Seyfedinova, N/A, Doctor, Doctor, Research Scientist, Research Laboratory of Digestive Cancer, Almazov Medical Research Centre, Akkuratova Av. 2, Saint-Petersburg 197341, Russia. seysabina000@gmail.com |
| Key Words |
Endoscopic ultrasound-guided, fine-needle aspiration; Pancreatic cancer; Pancreatic cysts; RNA extraction; Through-the-needle biopsy; Next-generation sequencing |
| Core Tip |
In this study, the use of RNA from endoscopic ultrasound-guided, fine-needle aspiration (EUS-FNA) samples of cystic and solid pancreatic neoplasms, including samples of low-quality RNA, was demonstrated. The process of sample preparation from EUS-FNA material is detailed along with the necessary criteria for assessing RNA quality for effective molecular biological analysis. Issues regarding the dependence of RNA quality on the type of neoplasm and material are identified. Modification of the library preparation protocol made it possible to obtain libraries for samples with RNA integrity number equivalent values of three and above, meeting the quality criteria for purity and concentration. |
| Publish Date |
2024-06-15 02:49 |
| Citation |
Seyfedinova SS, Freylikhman OA, Sokolnikova PS, Samochernykh KA, Kostareva AA, Kalinina OV, Solonitsyn EG. Fine-needle aspiration technique under endoscopic ultrasound guidance: A technical approach for RNA profiling of pancreatic neoplasms. World J Gastrointest Oncol 2024; 16(6): 2663-2672 |
| URL |
https://www.wjgnet.com/1948-5204/full/v16/i6/2663.htm |
| DOI |
https://dx.doi.org/10.4251/wjgo.v16.i6.2663 |
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