Publication Name	World Journal of Gastroenterology
Manuscript ID	9203
Country	China

Received	2014-01-27 10:26
Peer-Review Started	2014-01-27 15:29
To Make the First Decision	2014-02-28 21:13
Return for Revision	2014-03-06 19:59
Revised	2014-03-30 23:39
Second Decision	2014-06-26 17:05
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2014-06-26 17:23
Articles in Press	2014-06-26 17:52
Publication Fee Transferred
Edit the Manuscript by Language Editor	2014-08-13 07:08
Typeset the Manuscript	2014-09-12 08:35
Publish the Manuscript Online	2014-10-01 20:25

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access
Copyright
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Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Cell Biology
Manuscript Type	Autobiography
Article Title	Reversible immortalization of human hepatocytes mediated by retroviral transfer and site-specific recombination
Manuscript Source	Unsolicited Manuscript
All Author List	Fan-Ying Meng, Li Liu, Feng-Hui Yang, Chun-You Li, Jun Liu and Ping Zhou
Funding Agency and Grant Number	Funding Agency Grant Number Major Scientific and Technological Project of Shandong Province 201221019 Cisco Clinical Oncology Research Fund and Bayer Schering Cancer Research Fund Y-B2012-011
Corresponding Author	Jun Liu, MD, PhD, Division of Hepato-Biliary-Pancreatic Surgery, Institute of Organ Transplantation, Shandong Provincial Hospital Affiliated to Shandong University, Jingwuwei 7 Road 324, Jinan 250021, Shandong Province, China. 963cat@sohu.com
Key Words	Hepatocyte; Primary human hepatocytes; Reversible immortalization; Hepatocyte isolation; SV40T
Core Tip	It is meaningful to establish reversibly immortalized human hepatocytes which can be economically grown in tissue culture. Toward this goal, we successfully established a method for the reversible immortalization of human hepatocytes using Cre/loxP site-specific recombination, which may offer a good and safe source of hepatocytes for the bioartificial liver (BAL) system in the near future. If a sufficient number of human hepatocytes can be used, these extracorporeal devices will serve as successful “bridge-to-transplant”therapies. With the progress made in bioreactor development, the next-generation BAL system could reach the level of artificial kidney and save more patients.
Publish Date	2014-10-01 20:25
Citation	Meng FY, Liu L, Yang FH, Li CY, Liu J, Zhou P. Reversible immortalization of human hepatocytes mediated by retroviral transfer and site-specific recombination. World J Gastroenterol 2014; 20(36): 13119-13126
URL	http://www.wjgnet.com/1007-9327/full/v20/i36/13119.htm
DOI	http://dx.doi.org/10.3748/wjg.v20.i36.13119

Full Article (PDF)	WJG-20-13119.pdf
Full Article (Word)	WJG-20-13119.doc
Manuscript File	9203-Review.doc
Answering Reviewers	9203-Answering reviewers.pdf
Copyright License Agreement	9203-Copyright assignment.pdf
Non-Native Speakers of English Editing Certificate	9203-Language certificate.PDF
Peer-review Report	9203-Peer review.pdf
Scientific Misconduct Check	9203-CorssCheck.jpg
Scientific Editor Work List	9203-Scientific editor work list.pdf