Publication Name	World Journal of Diabetes
Manuscript ID	92991
Country	China

Received	2024-02-15 07:24
Peer-Review Started	2024-02-15 07:25
To Make the First Decision
Return for Revision	2024-04-23 06:06
Revised	2024-05-09 14:17
Second Decision	2024-06-11 06:55
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2024-06-18 08:44
Articles in Press	2024-06-18 08:44
Publication Fee Transferred	2024-06-19 01:54
Edit the Manuscript by Language Editor
Typeset the Manuscript	2024-06-29 14:51
Publish the Manuscript Online	2024-07-26 06:56

ISSN	1948-9358 (online)
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Category	Genetics & Heredity
Manuscript Type	Basic Study
Article Title	Functional analysis of the novel mitochondrial tRNATrp and tRNASer(AGY) variants associated with type 2 diabetes mellitus
Manuscript Source	Unsolicited Manuscript
All Author List	Yu Ding, Xue-Jiao Yu, Qin-Xian Guo and Jian-Hang Leng
ORCID	Author(s) ORCID Number Yu Ding http://orcid.org/0000-0003-1246-2563
Funding Agency and Grant Number	Funding Agency Grant Number Hangzhou Joint Fund of the Zhejiang Provincial Natural Science Foundation of China LHZY24H020002 Hangzhou Municipal Health Commission ZD20220010 Quzhou Bureau of Science and Technology 2022K51
Corresponding Author	Yu Ding, MD, Associate Professor, Central Laboratory, Hangzhou First People's Hospital, No. 261 Huansha Road, Hangzhou 310006, Zhejiang Province, China. dingyu_zj@126.com
Key Words	Type 2 diabetes mellitus; Mitochondrial tRNA genes; Novel variants; Oxidative stress; Mitochondrial dysfunctions
Core Tip	We established cytoplasmic hybrid (cybrid) cells with m.A5514G and m.C12237T variants, and control cells without these variants. The m.A5514G variant decreased mt-tRNATrp stability, whereas the m.C12237T variant did not alter the stability of mt-tRNASer(AGY). More severe mitochondrial dysfunction was observed in mutant cybrids than in control cells, indicating that the m.A5514G variant impaired mt-tRNATrp metabolism and mitochondrial functions and increased cellular oxidative stress, which play central roles in type 2 diabetes mellitus (T2DM) progression. By contrast, the m.C12237T variant acted as a modifier of the m.A5514G variant. Our study provides novel insight into the pathophysiology of maternally transmitted T2DM caused by novel mt-tRNA variants.
Publish Date	2024-07-26 06:56
Citation	<p>Ding Y, Yu XJ, Guo QX, Leng JH. Functional analysis of the novel mitochondrial tRNATrp and tRNASer(AGY) variants associated with type 2 diabetes mellitus. <i>World J Diabetes</i> 2024; 15(8): 1753-1763</p>
URL	https://www.wjgnet.com/1948-9358/full/v15/i8/1753.htm
DOI	https://dx.doi.org/10.4239/wjd.v15.i8.1753

Full Article (PDF)	WJD-15-1753-with-cover.pdf
Manuscript File	92991_Auto_Edited-YJP.docx
Answering Reviewers	92991-answering-reviewers.pdf
Audio Core Tip	92991-audio.m4a
Biostatistics Review Certificate	92991-biostatistics-statement.pdf
Conflict-of-Interest Disclosure Form	92991-conflict-of-interest-statement.pdf
Copyright License Agreement	92991-copyright-assignment.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	92991-foundation-statement.pdf
Institutional Review Board Approval Form or Document	92991-institutional-review-board-statement.pdf
Non-Native Speakers of English Editing Certificate	92991-non-native-speakers.pdf
Peer-review Report	92991-peer-reviews.pdf
Scientific Misconduct Check	92991-scientific-misconduct-check.png
Scientific Editor Work List	92991-scientific-editor-work-list.pdf
CrossCheck Report	92991-crosscheck-report.pdf