Publication Name	World Journal of Gastroenterology
Manuscript ID	9313
Country	China

Received	2014-01-30 10:29
Peer-Review Started	2014-02-08 17:01
To Make the First Decision	2014-02-28 14:53
Return for Revision	2014-03-13 09:16
Revised	2014-08-28 06:32
Second Decision	2014-10-15 12:32
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2014-10-15 17:40
Articles in Press	2014-10-15 17:56
Publication Fee Transferred
Edit the Manuscript by Language Editor	2014-11-04 07:17
Typeset the Manuscript	2014-12-15 19:46
Publish the Manuscript Online	2015-01-06 09:08

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access
Copyright
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Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Virology
Manuscript Type	Autobiography
Article Title	Tricistronic hepatitis C virus subgenomic replicon expressing double transgenes
Manuscript Source	Unsolicited Manuscript
All Author List	Xin Cheng, Xiang-Cui Gao, Jun-Ping Wang, Xin-Ying Yang, Yan Wang, Bao-Sheng Li, Fu-Biao Kang, Hai-Jun Li, Yue-Min Nan and Dian-Xing Sun
Funding Agency and Grant Number	Funding Agency Grant Number Study on Prevention and Control of Viral Hepatitis in the Key Program for Science and Technology Development of Hebei Province 10276102D
Corresponding Author	Dian-Xing Sun, MD, Professor of Medicine, The Liver Disease Diagnosis and Treatment Center of PLA, Bethune International Peace Hospital, 398 West Zhongshan Road, Shijiazhuang 050082, Hebei Province, China. sundianxing@hotmail.com
Key Words	Hepacivirus; Replicon; Internal ribosome entry site; Tricistronic expression
Core Tip	Two sequential RNA-binding motif protein 3 internal ribosome entry sites (IRESs) of 22 nucleotides were used to construct a tricistronic hepatitis C virus (HCV) replicon, initiating translation of humanized Renilla luciferase and HCV non-structural gene, along with HCV authentic IRES initiating translation of neomycin resistance gene. Intracellular HCV replicon RNA and expression of inserted foreign genes and HCV non-structural gene in cells transiently and stably transfected with tricistronic replicon RNA were comparable to those in cells transfected with traditional bicistronic HCV replicons. The novel replicon could be applied to evaluate replication inhibition efficacy of anti-HCV agents, except for interferon-?2b, which might be attributed to suppressed interferon response pathway.
Publish Date	2015-01-06 09:08
Citation	Cheng X, Gao XC, Wang JP, Yang XY, Wang Y, Li BS, Kang FB, Li HJ, Nan YM, Sun DX. Tricistronic hepatitis C virus subgenomic replicon expressing double transgenes. World J Gastroenterol 2014; 20(48): 18284-18295
URL	http://www.wjgnet.com/1007-9327/full/v20/i48/18284.htm
DOI	http://dx.doi.org/10.3748/wjg.v20.i48.18284

Full Article (PDF)	WJG-20-18284.pdf
Full Article (Word)	WJG-20-18284.doc
Manuscript File	9313-Review.doc
Answering Reviewers	9313-Answering reviewers.pdf
Copyright License Agreement	9313-Copyright assignment.pdf
Non-Native Speakers of English Editing Certificate	9313-Language certificate.pdf
Peer-review Report	9313-Peer reviews.pdf
Scientific Misconduct Check	9313-CrossCheck.jpg
Scientific Editor Work List	9313-Scientific editor work list.pdf