| ISSN |
2220-3249 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Virology |
| Manuscript Type |
Retrospective Study |
| Article Title |
Diagnosis of West Nile virus infections: Evaluation of different laboratory methods
|
| Manuscript Source |
Invited Manuscript |
| All Author List |
Tatjana Vilibic-Cavlek, Maja Bogdanic, Vladimir Savic, Zeljka Hruskar, Ljubo Barbic, Vladimir Stevanovic, Ljiljana Antolasic, Ljiljana Milasincic, Dario Sabadi, Gorana Miletic, Ivona Coric, Anna Mrzljak, Eddy Listes and Giovanni Savini |
| ORCID |
|
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| the Croatian Science Foundation |
IP-2016-06-7456: CRONEUROARBO |
| the European Union NextGenerationEU project supported by the Ministry of Science and Education of the Republic of Croatia |
NPOO 1 of Croatian Veterinary Institute: FLAVIR |
|
| Corresponding Author |
Tatjana Vilibic-Cavlek, MD, Full Professor, Department of Virology, Croatian Institute of Public Health, Rockefellerova 3, Zagreb 10000, Croatia. tanja.vilibic@gmail.com |
| Key Words |
West Nile virus; Reverse-transcriptase polymerase chain reaction; Serology; IgG avidity; Cross-reactivity |
| Core Tip |
We analyzed different diagnostic methods in patients with West Nile virus (WNV) neuroinvasive disease and asymptomatic seropositive individuals. The WNV RNA detection rate was significantly higher in the urine/serum than in cerebrospinal fluid (CSF). According to the sampling time (≤ 7 days, 8-14 days, ≥ 15 days), the RT-PCR positive detection rates were 36.4/31.2/25.0% in the CSF, and 29.4/66.6/62.5% in urine. The RT-PCR cycle threshold (Ct) values were significantly lower in urine than in CSF/serum samples. Using ELISA, positive immunoglobulin M (IgM) detection rates in serum/CSF were 84.3/62.5% in samples collected ≤ 7 days, 100/73.9% in samples collected 8-14 days, and 100/100% in samples collected ≥ 15 days after symptoms onset. Immunoglobulin G (IgG) detection rates in serum/CSF were 9.3/28.1% in samples ≤ 7 days, 71.1/26.1% in samples 8-14 days, and 100/57.1% in samples collected ≥ 15 days. The correlation between ELISA/IgG avidity was negative for IgM and positive for IgG. No correlation was observed between ELISA IgG/VNT. |
| Publish Date |
2024-10-18 06:31 |
| Citation |
<p>Vilibic-Cavlek T, Bogdanic M, Savic V, Hruskar Z, Barbic L, Stevanovic V, Antolasic L, Milasincic L, Sabadi D, Miletic G, Coric I, Mrzljak A, Listes E, Savini G. Diagnosis of West Nile virus infections: Evaluation of different laboratory methods. <i>World J Virol</i> 2024; 13(4): 95986</p> |
| URL |
https://www.wjgnet.com/2220-3249/full/v13/i4/95986.htm |
| DOI |
https://dx.doi.org/10.5501/wjv.v13.i4.95986 |
