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Articles Published Processes
3/6/2025 10:23:12 AM | Browse: 98 | Download: 142
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Received |
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2024-08-22 09:30 |
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Peer-Review Started |
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2024-06-20 08:16 |
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To Make the First Decision |
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Return for Revision |
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2024-11-06 07:33 |
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Revised |
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2024-11-19 10:12 |
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Second Decision |
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2024-12-23 02:41 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Executive Editor-in-Chief |
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2024-12-23 08:36 |
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Articles in Press |
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2024-12-23 08:36 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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Typeset the Manuscript |
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2024-12-30 04:42 |
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Publish the Manuscript Online |
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2025-03-06 10:23 |
ISSN |
2222-0682 (online) |
Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/ |
Copyright |
© The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved. |
Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
Website |
http://www.wjgnet.com |
Category |
Cell Biology |
Manuscript Type |
Basic Study |
Article Title |
Selective procedure for the instant identification of cellular apoptosis induced by natural products
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Manuscript Source |
Invited Manuscript |
All Author List |
Ying-Yu Cui |
ORCID |
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Funding Agency and Grant Number |
Funding Agency |
Grant Number |
2023 Key R&D Projects of Tongji University |
150029607160-24323 |
Industrial Key Program Foundation of Guangdong Province: R&D of Natural Novel Anticancer Drugs |
2004B10401033 |
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Corresponding Author |
Ying-Yu Cui, Associate Professor, PhD, Department of Cell Biology, Institute of Medical Genetics, State Key Laboratory of Cardiology, Tongji University School of Medicine, No. 500 Zhennan Road, Putuo District, Shanghai 200331, China. yycui@tongji.edu.cn |
Key Words |
Apoptosis identification; Fluorescence microscopy; DNA gel electrophoresis; Flow cytometry; Pinus massoniana bark extract; One transient cell processing procedure |
Core Tip |
According to the global green development hypothesis, the identification of cell apoptosis induced by natural products is a research hotspot. However, routine laboratory identification of apoptosis suffers from experimental errors and poor repeatability. Herein, we explored the simultaneous morphological, biochemical and molecular identification of apoptosis, while reducing experimental errors. We established a protocol to instantly identify apoptosis following natural product treatment, termed one transient cell processing procedure (OTCPP), comprising eleven steps. It could identify apoptosis of cultured LoVo cells in a little as 4 days. The OTCPP procedure is easy, rapid and high efficient, especially for researchers in developing countries. |
Publish Date |
2025-03-06 10:23 |
Citation |
<p>Cui YY. Selective procedure for the instant identification of cellular apoptosis induced by natural products. <i>World J Methodol</i> 2025; 15(3): 98201</p> |
URL |
https://www.wjgnet.com/2222-0682/full/v15/i3/98201.htm |
DOI |
https://dx.doi.org/10.5662/wjm.v15.i3.98201 |
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