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Publication Name World Journal of Methodology
Manuscript ID 98201
Country China
Received
2024-08-22 09:30
Peer-Review Started
2024-06-20 08:16
To Make the First Decision
Return for Revision
2024-11-06 07:33
Revised
2024-11-19 10:12
Second Decision
2024-12-23 02:41
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief
2024-12-23 08:36
Articles in Press
2024-12-23 08:36
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript
2024-12-30 04:42
Publish the Manuscript Online
2025-03-06 10:23
ISSN 2222-0682 (online)
Open Access This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Copyright © The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
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Publisher Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website http://www.wjgnet.com
Category Cell Biology
Manuscript Type Basic Study
Article Title Selective procedure for the instant identification of cellular apoptosis induced by natural products
Manuscript Source Invited Manuscript
All Author List Ying-Yu Cui
ORCID
Author(s) ORCID Number
Ying-Yu Cui http://orcid.org/0000-0002-6324-0795
Funding Agency and Grant Number
Funding Agency Grant Number
2023 Key R&D Projects of Tongji University 150029607160-24323
Industrial Key Program Foundation of Guangdong Province: R&D of Natural Novel Anticancer Drugs 2004B10401033
Corresponding Author Ying-Yu Cui, Associate Professor, PhD, Department of Cell Biology, Institute of Medical Genetics, State Key Laboratory of Cardiology, Tongji University School of Medicine, No. 500 Zhennan Road, Putuo District, Shanghai 200331, China. yycui@tongji.edu.cn
Key Words Apoptosis identification; Fluorescence microscopy; DNA gel electrophoresis; Flow cytometry; Pinus massoniana bark extract; One transient cell processing procedure
Core Tip According to the global green development hypothesis, the identification of cell apoptosis induced by natural products is a research hotspot. However, routine laboratory identification of apoptosis suffers from experimental errors and poor repeatability. Herein, we explored the simultaneous morphological, biochemical and molecular identification of apoptosis, while reducing experimental errors. We established a protocol to instantly identify apoptosis following natural product treatment, termed one transient cell processing procedure (OTCPP), comprising eleven steps. It could identify apoptosis of cultured LoVo cells in a little as 4 days. The OTCPP procedure is easy, rapid and high efficient, especially for researchers in developing countries.
Publish Date 2025-03-06 10:23
Citation <p>Cui YY. Selective procedure for the instant identification of cellular apoptosis induced by natural products. <i>World J Methodol</i> 2025; 15(3): 98201</p>
URL https://www.wjgnet.com/2222-0682/full/v15/i3/98201.htm
DOI https://dx.doi.org/10.5662/wjm.v15.i3.98201
Full Article (PDF) WJM-15-98201-with-cover.pdf
Manuscript File 98201_Auto_Edited_102033.docx
Answering Reviewers 98201-answering-reviewers.pdf
Audio Core Tip 98201-audio.m4a
Biostatistics Review Certificate 98201-biostatistics-statement.pdf
Conflict-of-Interest Disclosure Form 98201-conflict-of-interest-statement.pdf
Copyright License Agreement 98201-copyright-assignment.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s) 98201-foundation-statement.pdf
Institutional Review Board Approval Form or Document 98201-institutional-review-board-statement.pdf
Non-Native Speakers of English Editing Certificate 98201-non-native-speakers.pdf
Peer-review Report 98201-peer-reviews.pdf
Scientific Misconduct Check 98201-scientific-misconduct-check.png
Scientific Editor Work List 98201-scientific-editor-work-list.pdf
CrossCheck Report 98201-crosscheck-report.pdf