| ISSN |
1948-0210 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Cell Biology |
| Manuscript Type |
Basic Study |
| Article Title |
Immortalization of epidural fat-derived mesenchymal stem cells: In vitro characterization and adipocyte differentiation potential
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Seoung-Woo Lee, Young-Ju Lim, Hee-Yeon Kim, Wansoo Kim, Wook-Tae Park, Min-Jung Ma, Junho Lee, Min-Soo Seo, Young In Kim, Sangbum Park, Seong-Kyoon Choi and Gun Woo Lee |
| ORCID |
|
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Centers of Research Excellence - Regional Leading Research |
RS-2023-00219725 |
| National Research Foundation of Korea |
2022R1C1C1005410, 00219725 |
| Korea Health Technology R&D Project through the Korea Health Industry Development Institute, Ministry of Health & Welfare, Republic of Korea |
RS-2023-00305198 |
| DGIST R&D Program of the Ministry of Science and ICT 24-NT-01 |
060007 |
| NIH |
R01AR083086 |
|
| Corresponding Author |
Gun Woo Lee, Associate Professor, MD, PhD, Department of Orthopedic Surgery, Yeungnam University College of Medicine, No. 170 Hyunchung-ro, Namgu, Daegu 42415, South Korea. gwlee1871@gmail.com |
| Key Words |
Immortalization; Transfection; Stem cell; Epidural fat; Proteomics |
| Core Tip |
According to prior research, epidural fat-derived mesenchymal stem cells (EF-MSCs) are potential candidates to treat neurodegenerative diseases. However, the clinical implication of EF-MSCs has encountered many limitations including difficulty in isolating the fat from the epidural region and limited proliferation ability of the stem cells. Therefore, precise biomolecular characteristics of the EF-MSC are still unclear. To overcome these problems, we immortalized EF-MSCs through lentiviral transduction, and these stable EF-MSCs were analyzed by phenotypical interpretation and proteomics. In the present study, immortalized EF-MSCs demonstrated notably increased lifespan and adipogenic potential. These findings suggest that stable EF-MSCs could be potential tools for developing effective regenerative medicine strategies. |
| Publish Date |
2025-01-20 02:39 |
| Citation |
Lee SW, Lim YJ, Kim HY, Kim W, Park WT, Ma MJ, Lee J, Seo MS, Kim YI, Park S, Choi SK, Lee GW. Immortalization of epidural fat-derived mesenchymal stem cells: In vitro characterization and adipocyte differentiation potential. World J Stem Cells 2025; 17(1): 98777 |
| URL |
https://www.wjgnet.com/1948-0210/full/v17/i1/98777.htm |
| DOI |
https://dx.doi.org/10.4252/wjsc.v17.i1.98777 |
