| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Virology |
| Manuscript Type |
Basic Study |
| Article Title |
High persistence rate of hepatitis B virus in a hydrodynamic injection-based transfection model in C3H/HeN mice
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Xiu-Hua Peng, Xiao-Nan Ren, Li-Xiang Chen, Bi-Sheng Shi, Chun-Hua Xu, Zhong Fang, Xue Liu, Jie-Liang Chen, Xiao-Nan Zhang, Yun-Wen Hu and Xiao-Hui Zhou |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Shanghai Municipal Fund for Science and Technology Development |
13140902200 |
| Shanghai Municipal Fund for Science and Technology Development |
12140900300 |
| National Basic Research Program of China（973 Program） |
2012CB519005 |
|
| Corresponding Author |
Xiao-Hui Zhou, MD, PhD, Associate Professor, Shanghai Public Health Clinical Center, Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, Fudan University, 2901 Caolang Road, Jin Shan District, Shanghai 201508, China. zhouxiaohui@shaphc.org |
| Key Words |
Mouse; Liver; Viral persistence; Hydrodynamic injection; Hepatitis B virus |
| Core Tip |
In the classic hepatitis B virus (HBV) hydrodynamic injection (HI) model using C57BL/6 mice, only about 30% of the injected mice carried HBV for more than 12 wk. Here we injected the pAAV-HBV1.2 plasmid into C3H/HeN mice and observed that the hepatitis B surface antigen, hepatitis B e antigen and viral DNA persisted even up to 46 wk in about 90% of the hydrodynamically injected mice. Applying interferon-? or entecavir in this HI model decreased HBV DNA in vivo. Hence, C3H/HeN is a suitable mouse strain for the persistent HBV HI model, which might be useful for chronic hepatitis B research and therapeutic drug development. |
| Publish Date |
2015-03-30 14:23 |
| Citation |
Peng XH, Ren XN, Chen LX, Shi BS, Xu CH, Fang Z, Liu X, Chen JL, Zhang XN, Hu YW, Zhou XH. High persistence rate of hepatitis B virus in a hydrodynamic injection-based transfection model in C3H/HeN mice. World J Gastroenterol 2015; 21(12): 3527-3536 |
| URL |
http://www.wjgnet.com/1007-9327/full/v21/i12/3527.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v21.i12.3527 |
