Publication Name	World Journal of Gastroenterology
Manuscript ID	13904
Country	Germany

Received	2014-09-07 17:03
Peer-Review Started	2014-09-07 19:03
To Make the First Decision	2014-10-14 16:49
Return for Revision	2014-10-20 09:55
Revised	2014-11-03 20:44
Second Decision	2014-11-24 11:57
Accepted by Journal Editor-in-Chief	2014-11-25 19:07
Accepted by Company Editor-in-Chief	2014-12-08 16:38
Articles in Press	2014-12-08 17:15
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2015-03-10 13:45
Publish the Manuscript Online	2015-03-19 16:57

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access	This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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Category	Gastroenterology & Hepatology
Manuscript Type	Observational Study
Article Title	Lack of correlation between Treg quantification assays in inflammatory bowel disease patients
Manuscript Source	Unsolicited Manuscript
All Author List	Gunnar Brandhorst, Darinka Todorova Petrova, Sebastian Weigand, Christoph Eberle, Nicolas von Ahsen, Jessica Schmitz, Frank Christian Schultze, Dirk Raddatz, Michael Karaus, Michael Oellerich and Philip D Walson
Funding Agency and Grant Number	Funding Agency Grant Number Medical Faculty of the University of Goettingen of Germany German Research Foundation Open Access Publication Funds of the Goettingen
Corresponding Author	Darinka Todorova Petrova, PhD, Department of Clinical Chemistry, University Medical Center, Robert-Koch-Str. 40, 37075 Goettingen, Germany. darinka.petrova@med.uni-goettingen.de
Key Words	Regulatory T-cells; Inflammatory bowel disease; Crohn´s disease; Ulcerative colitis; Method comparison
Core Tip	In our study neither regulatory T-cells (Tregs) percentages measured by flow cytometry defined as CD4+CD25highCD127lowFOXP3+ cells in peripheral blood mononuclear cells or by real-time PCR measured as forkhead box P3 promoter demethylation in genomic DNA correlated with self-reported inflammatory bowel disease activity. This suggests that either Treg-related immune function or the clinical scores did not accurately reflect actual disease activity. We conclude that natural and induced Tregs are not equally detected by the assays applied.
Publish Date	2015-03-19 16:57
Citation	Brandhorst G, Petrova DT, Weigand S, Eberle C, von Ahsen N, Schmitz J, Schultze FC, Raddatz D, Karaus M, Oellerich M, Walson PD. Lack of correlation between Treg quantification assays in inflammatory bowel disease patients. World J Gastroenterol 2015; 21(11): 3325-3329
URL	http://www.wjgnet.com/1007-9327/full/v21/i11/3325.htm
DOI	http://dx.doi.org/10.3748/wjg.v21.i11.3325

Full Article (PDF)	WJG-21-3325.pdf
Full Article (Word)	WJG-21-3325.doc
Manuscript File	13904-Review.docx
Answering Reviewers	13904-Answering reviewers.pdf
Copyright License Agreement	13904-Copyright assignment.pdf
Peer-review Report	13904-Peer review(s).pdf
Scientific Misconduct Check	13904-CrossCheck.jpg
Scientific Editor Work List	13904-Scientific editor work list.pdf