Publication Name	World Journal of Virology
Manuscript ID	16610
Country	India

Received	2015-01-25 12:25
Peer-Review Started	2015-01-27 20:30
To Make the First Decision	2015-03-06 10:11
Return for Revision	2015-03-15 17:50
Revised	2015-03-23 15:51
Second Decision	2015-04-30 16:28
Accepted by Journal Editor-in-Chief	2015-05-01 14:11
Accepted by Company Editor-in-Chief	2015-05-08 16:33
Articles in Press	2015-05-08 16:33
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2015-08-09 14:20
Publish the Manuscript Online	2015-08-13 10:30

ISSN	2220-3249 (online)
Open Access	This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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Category	Virology
Manuscript Type	Review
Article Title	Next-generation sequencing in clinical virology: Discovery of new viruses
Manuscript Source	Invited Manuscript
All Author List	Sibnarayan Datta, Raghvendra Budhauliya, Bidisha Das, Soumya Chatterjee, Vanlalhmuaka and Vijay Veer
Funding Agency and Grant Number	Funding Agency Grant Number Defence Research and Development Organization Ministry of Defence, Government of India
Corresponding Author	Sibnarayan Datta, PhD, Molecular Virology Laboratory, Defence Research Laboratory (DRDO), Post bag No. 2, Tezpur, Assam, PIN-784001, India. sndatta1978@gmail.com
Key Words	PCR; Next-generation sequencers; Virus discovery; Sequence-independent single-primer amplif¬ication; Virus discovery based on cDNA-AFLP; Rolling circle amplification; Metagenomics
Core Tip	Rapid development and commercial availability of next-generation sequencers (NGS) systems have dramatically changed almost every field of biological research, especially microbiology and metagenomics. Different NGS systems have been adapted and used for numerous applications in virology too. These systems are capable of rapidly sequencing and analyzing a complex mixture of nucleic acid templates, in a massively parallel fashion, making them ideal tools for viral metagenomics and discovery. This manuscript reviews the prevailing NGS technologies, their application in virus discovery to serve as a guide for the readers, working in the field of virology, public health and in biothreat mitigation programs.
Publish Date	2015-08-13 10:30
Citation	Datta S, Budhauliya R, Das B, Chatterjee S, Vanlalhmuaka, Veer V. Next-generation sequencing in clinical virology: Discovery of new viruses. World J Virol 2015; 4(3): 265-276
URL	http://www.wjgnet.com/2220-3249/full/v4/i3/265.htm
DOI	http://dx.doi.org/10.5501/wjv.v4.i3.265

Full Article (PDF)	WJV-4-265.pdf
Full Article (Word)	WJV-4-265.doc
Manuscript File	16610-Review.docx
Answering Reviewers	16610-Answering reviewers.pdf
Audio Core Tip	16610-Audio core tip.m4a
Conflict-of-Interest Disclosure Form	16610-Conflict-of-interest statement.pdf
Copyright License Agreement	16610-Copyright assignment.pdf
Peer-review Report	16610-Peer-review(s).pdf
Journal Editor-in-Chief Review Report	16610-Journal editor-in-chief review report.pdf
Scientific Misconduct Check	16610-Scientific misconduct check.pdf
Scientific Editor Work List	16610-Scientific editor work list.pdf