| Category |
Genetics & Heredity |
| Manuscript Type |
Autobiography |
| Article Title |
Reference genes for quantitative RT-PCR data in gastric tissues and cell lines
|
| Manuscript Source |
Invited Manuscript |
| All Author List |
Fernanda Wisnieski, Danielle Queiroz Calcagno, Mariana Ferreira Leal, Leonardo Caires dos Santos, Carolina de Oliveira Gigek, Elizabeth Suchi Chen, Thaís Brilhante Pontes, Paulo Pimentel Assumpção, Mônica Barauna de Assumpção, Sâmia Demachki, Rommel Rodríguez Burbano and Marília de Arruda Cardoso Smith |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) to Wisnieski F and Gigek CO |
|
| Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) to Burbano RR and Smith MAC |
|
| Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) to Wisnieski F, Calcagno DQ, Leal M, Pontes TB and Smith MAC as grants and fellowship awards |
|
|
| Corresponding Author |
Fernanda Wisnieski, PhD, Genetics Division, Department of Morphology and Genetic, Federal University of São Paulo, São Paulo 04023900, Brazil. fernandawis@yahoo.com.br |
| Key Words |
Gastric cancer; Reference gene; Normalization; Gene expression; Quantitative real-time polymerase chain reaction |
| Core Tip |
Gene expression studies have revealed much about the molecular basis of gastric cancer. However, the normalization of expression data using reference genes without validation may undermine the results. In the present study, we evaluated the suitability of possible reference genes in gastric tissues and cell lines. To our knowledge, our study is the first to determine and validate reference genes for gastric samples in a Western population. In addition, the inclusion of normal gastric tissues from patients without cancer in determining the best reference genes is original in the literature. |
| Publish Date |
2013-11-16 18:29 |
| Citation |
Wisnieski F, Calcagno DQ, Leal MF, Santos LC, Gigek CO, Chen ES, Pontes TB, Assumpção PP, Assumpção MB, Demachki S, Burbano RR, Smith MAC. Reference genes for quantitative RT-PCR data in gastric tissues and cell lines. World J Gastroenterol 2013; 19(41): 7121-7128 |
| URL |
http://www.wjgnet.com/1007-9327/full/v19/i41/7121.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v19.i41.7121 |
