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6/13/2016 9:14:00 AM | Browse: 918 | Download: 1486
Publication Name World Journal of Gastroenterology
Manuscript ID 23084
Country/Territory China
Received
2015-12-03 08:39
Peer-Review Started
2015-12-04 08:51
To Make the First Decision
2015-12-21 15:16
Return for Revision
2015-12-22 09:54
Revised
2016-04-06 13:15
Second Decision
2016-04-13 07:54
Accepted by Journal Editor-in-Chief
2016-04-13 16:23
Accepted by Company Editor-in-Chief
2016-04-20 14:15
Articles in Press
2016-04-20 14:15
Publication Fee Transferred
Edit the Manuscript by Language Editor
2016-05-14 01:28
Typeset the Manuscript
2016-05-24 13:54
Publish the Manuscript Online
2016-06-13 09:14
ISSN 1007-9327 (print) and 2219-2840 (online)
Open Access This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Copyright © The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
Article Reprints For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website http://www.wjgnet.com
Category Oncology
Manuscript Type Basic Study
Article Title Tumor-specific expression of shVEGF and suicide gene as a novel strategy for esophageal cancer therapy
Manuscript Source Unsolicited Manuscript
All Author List Ting Liu, Hai-Jun Wu, Yu Liang, Xu-Jun Liang, Hui-Chao Huang, Yan-Zhong Zhao, Qing-Chuan Liao, Ya-Qi Chen, Ai-Min Leng, Wei-Jian Yuan, Gui-Ying Zhang, Jie Peng and Yong-Heng Chen
Funding Agency and Grant Number
Funding Agency Grant Number
National Natural Science Foundation of China 81372904
National Natural Science Foundation of China 81272971
National Natural Science Foundation of China 81272735
National Natural Science Foundation of China 30800518
Science and Technology Department of Hunan Province 2010CK3013
Corresponding Author Yong-Heng Chen, MD, PhD, Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, XiangYa Hospital, Central South University, No. 87 Xiangya Road, Changsha 410008, Hunan Province, China. yonghenc@163.com
Key Words Esophageal cancer; Suicide gene; RNA interference; Vascular endothelial growth factor; Nanoparticles
Core Tip Esophageal cancer is a highly aggressive neoplasm with poor prognosis and low survival rates. In this study, an expression vector carrying a fusion suicide gene (yCDglyTK) and shRNA against vascular endothelial growth factor (VEGF) was constructed and delivered into EC9706 esophageal cancer cells by calcium phosphate nanoparticles (CPNP). To achieve tumor selectivity, the expression of the fusion suicide gene was driven by a tumor-specific human telomerase reverse transcriptase promoter. Our results showed that the novel expression vector was efficiently introduced into EC9706 cells by CPNP, leading to cellular expression of yCDglyTK and decreased VEGF level. With exposure to 5-fluorocytosine, it exhibited strong anti-tumor effects against esophageal cancer both in vitro and in vivo. Combination of VEGF shRNA with the fusion suicide gene demonstrated strong anti-cancer effects. Our study provides a novel approach for esophageal cancer-targeted gene therapy.
Publish Date 2016-06-13 09:14
Citation Liu T, Wu HJ, Liang Y, Liang XJ, Huang HC, Zhao YZ, Liao QC, Chen YQ, Leng AM, Yuan WJ, Zhang GY, Peng J, Chen YH. Tumor-specific expression of shVEGF and suicide gene as a novel strategy for esophageal cancer therapy. World J Gastroenterol 2016; 22(23): 5342-5352
URL http://www.wjgnet.com/1007-9327/full/v22/i23/5342.htm
DOI http://dx.doi.org/10.3748/wjg.v22.i23.5342
Full Article (PDF) WJG-22-5342.pdf
Full Article (Word) WJG-22-5342.doc
Manuscript File 23084-Review.docx
Answering Reviewers 23084-Answering reviewers.pdf
Audio Core Tip 23084-Audio core tip.m4a
Conflict-of-Interest Disclosure Form 23084-Conflict-of-interest statement.pdf
Copyright License Agreement 23084-Copyright assignment.pdf
Institutional Animal Care and Use Committee Approval Form or Document 23084-Institutional animal care and use committee statement.pdf
Institutional Review Board Approval Form or Document 23084-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate 23084-Language certificate.pdf
Peer-review Report 23084-Peer-review(s).pdf
Journal Editor-in-Chief Review Report 23084-Journal editor-in-chief review report.pdf
Scientific Misconduct Check 23084-Scientific misconduct check.pdf
Scientific Editor Work List 23084-Scientific editor work list.pdf