| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Peer-review started: February 25, 2016 |
| Copyright |
© The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Oncology |
| Manuscript Type |
Basic Study |
| Article Title |
Dissecting characteristics and dynamics of differentially expressed proteins during multistage carcinogenesis of human colorectal cancer
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Fang Peng, Ying Huang, Mao-Yu Li, Guo-Qing Li, Hui-Chao Huang, Rui Guan, Zhu-Chu Chen, Song-Ping Liang and Yong-Heng Chen |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| National Basic Research Program of China（973 Program） |
2011CB910704 |
| National Natural Science Foundation of China |
81372904 |
| National Natural Science Foundation of China |
81570537 |
| National Natural Science Foundation of China |
81272971 |
|
| Corresponding Author |
Yong-Heng Chen, MD, PhD, Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, XiangYa Hospital, Central South University, 87 Xiangya Road, Changsha 410008, Hunan Province, China. yonghengchen@gmail.com |
| Key Words |
Colorectal Cancer; Proteome; Biomarker; Carcinogenesis |
| Core Tip |
In this study, we used iTRAQ 2D LC-MS/MS analysis to identify differentially expressed proteins (DEPs) in the human colonic epithelial carcinogenic process using laser capture microdissection-purified colonic epithelial cells from normal colon, adenoma, carcinoma in situ and invasive carcinoma tissues. A total of 326 DEPs were identified. Four DEPs (DMBT1, S100A9, Galectin-10, and S100A8) with progressive alteration in the carcinogenic process were further validated using immunohistochemistry. The DEPs were involved in multiple biological processes including cell cycle, cell adhesion, translation, mRNA processing, and protein synthesis. These findings give insights into our understanding of the mechanisms of colorectal carcinogenesis and provide clues for further investigation of carcinogenesis and identification of biomarkers. |
| Publish Date |
2016-05-04 10:08 |
| Citation |
Peng F, Huang Y, Li MY, Li GQ, Huang HC, Guan R, Chen ZC, Liang SP, Chen YH. Dissecting characteristics and dynamics of differentially expressed proteins during multistage carcinogenesis of human colorectal cancer. World J Gastroenterol 2016; 22(18): 4515-4528 |
| URL |
http://www.wjgnet.com/1007-9327/full/v22/i18/4515.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v22.i18.4515 |
