Publication Name	World Journal of Gastroenterology
Manuscript ID	23610
Country	Germany

Received	2015-12-05 09:17
Peer-Review Started	2015-12-07 10:21
To Make the First Decision	2016-01-13 09:16
Return for Revision	2016-01-13 15:56
Revised	2016-01-24 02:48
Second Decision	2016-01-29 17:15
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2016-02-22 11:14
Articles in Press	2016-02-22 11:15
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2016-03-29 14:04
Publish the Manuscript Online	2016-04-19 09:42

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access	This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Copyright	© The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
Article Reprints	For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions	For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Gastroenterology & Hepatology
Manuscript Type	Basic Study
Article Title	Functional analysis and drug response to zinc and D-penicillamine in stable ATP7B mutant hepatic cell lines
Manuscript Source	Invited Manuscript
All Author List	Gursimran Chandhok, Judit Horvath, Annu Aggarwal, Mohit Bhatt, Andree Zibert and Hartmut HJ Schmidt
Funding Agency and Grant Number
Corresponding Author	Hartmut HJ Schmidt, MD, Professor, Klinik für Transplantationsmedizin, Universitätsklinikum Münster, Albert-Schweitzer-Campus 1, Gebäude A14, D-48149 Münster, Germany. hepar@ukmuenster.de
Key Words	ATP7B; D-penicillamine; Zinc; Mutations; Wilson disease; Therapy
Core Tip	Copper overload in Wilson disease (WD) is treated with anti-copper therapy. However, the effect of treatment has not been studied using human hepatic cells lacking the ATP7B copper transporter. Using a previously established ATP7B KO cell line, we generated stable mutant cell lines to study functional analysis and response to zinc (Zn) and D-penicillamine (DPA). All mutants showed individual response rates following copper and anti-copper treatment. Highest rescue from copper toxicity was observed after combined Zn and DPA treatment. The study provides novel insights into genotype-phenotype correlations and genotype-specific treatment of WD.
Publish Date	2016-04-19 09:42
Citation	Chandhok G, Horvath J, Aggarwal A, Bhatt M, Zibert A, Schmidt HHJ. Functional analysis and drug response to zinc and D-penicillamine in stable ATP7B mutant hepatic cell lines. World J Gastroenterol 2016; 22(16): 4109-4119
URL	http://www.wjgnet.com/1007-9327/full/v22/i16/4109.htm
DOI	http://dx.doi.org/10.3748/wjg.v22.i16.4109

Full Article (PDF)	WJG-22-4109.pdf
Full Article (Word)	WJG-22-4109.doc
Manuscript File	23610-Review.docx
Answering Reviewers	23610-Answering reviewers.pdf
Audio Core Tip	23610-Audio core tip.mp3
Conflict-of-Interest Disclosure Form	23610-Conflict-of-interest statement.pdf
Copyright License Agreement	23610-Copyright assignment.pdf
Supplementary Material	23610-Supplementary materials.pdf
Peer-review Report	23610-Peer-review(s).pdf
Scientific Misconduct Check	23610-Scientific misconduct check.pdf
Scientific Editor Work List	23610-Scientific editor work list.pdf