| ISSN |
2220-3249 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Virology |
| Manuscript Type |
Retrospective Study |
| Article Title |
Active tracking of rejected dried blood samples in a large program in Nigeria
|
| Manuscript Source |
Invited Manuscript |
| All Author List |
Auchi Inalegwu, Sunny Phillips, Rawlings Datir, Christopher Chime, Petronilla Ozumba, Samuel Peters, Obinna Ogbanufe, Charles Mensah, Alash’Le Abimiku, Patrick Dakum and Nicaise Ndembi |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| HHS/ Centers for Disease Control and Prevention |
5U2GGH000925-03 |
|
| Corresponding Author |
Nicaise Ndembi, Director, Institute of Human Virology, Pent House, Maina Court, 252 Herbert Macaulay Way, Central Business District, PO Box 9396 Garki, Abuja 900246, Federal Capital Territory, Nigeria. ndembinic@yahoo.fr |
| Key Words |
Human immunodeficiency virus; Prevention of mother-to-child transmission; Early infant diagnosis; Dried blood spot; Pre-analytical error; Sample rejection |
| Core Tip |
For early infant diagnosis of human immunodeficiency virus, the samples of choice are dried blood spots (DBS). DBS samples are received from over 100 health care centers at the Asokoro Laboratory Training Centre. When DBS arrives the laboratory, a technician receives the samples as well as all accompanying laboratory request forms and all relevant documentation. All routinely collected DBS samples are physically examined for quality and acceptability for molecular testing upon reception at the laboratory. Only samples that meet the laboratory acceptance criteria are usually tested. Samples which fail to meet the acceptance criteria are registered in the sample rejection logbook without being tested. All DBS samples accepted as fit-for-testing are electronically registered into the laboratory information management system (LIMS). The use of the LIMS reduces instances of transcriptional errors. DBS samples are processed using real-time PCR technology on the Cobas Taqman and Cobas ampliprep equipment. DBS spots are cut, eluted into solution, and then placed in the equipment where DNA extraction, amplification and detection is automatically carried out. Once results are ready, they are validated by the laboratory scientist for accuracy and completeness. If assay is judged to be a valid run, the assay is accepted with a click of a computer button. |
| Publish Date |
2016-05-06 13:48 |
| Citation |
Inalegwu A, Phillips S, Datir R, Chime C, Ozumba P, Peters S, Ogbanufe O, Mensah C, Abimiku A, Dakum P, Ndembi N. Active tracking of rejected dried blood samples in a large program in Nigeria. World J Virol 2016; 5(2): 73-81 |
| URL |
http://www.wjgnet.com/2220-3249/full/v5/i2/73.htm |
| DOI |
http://dx.doi.org/10.5501/wjv.v5.i2.73 |
