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9/15/2014 5:37:00 PM | Browse: 1008 | Download: 880
Publication Name World Journal of Stem Cells
Manuscript ID 2935
Country/Territory Singapore
Received
2013-03-26 14:26
Peer-Review Started
2013-03-28 09:28
To Make the First Decision
2013-04-16 09:14
Return for Revision
2013-04-18 14:21
Revised
2013-05-07 16:03
Second Decision
2013-06-08 14:12
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief
2013-06-10 04:10
Articles in Press
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript
2013-06-28 12:01
Publish the Manuscript Online
2013-07-25 08:11
ISSN 1948-0210 (online)
Open Access
Copyright
Article Reprints For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website http://www.wjgnet.com
Category Developmental Biology
Manuscript Type Autobiography
Article Title Nutrient supplemented serum-free medium increases cardiomyogenesis efficiency of human pluripotent stem cells
Manuscript Source Invited Manuscript
All Author List Sherwin Ting, Marti Lecina, Yau-Chi Chan, Hung Fat Tse, Shaul Reuveny and Steve KW Oh
Funding Agency and Grant Number
Funding Agency Grant Number
Science Technology and Research
Hong Hong Kong Research Grant Council Collaborative Research Fund HKU8/CRF/09
Theme-based Research Scheme T12-705/11
Corresponding Author Dr. Steve KW Oh, Principal Scientist, Bioprocessing Technology Institute, A*STAR (Agency for Science, Technology and Research), 20 Biopolis Way No. 06-01, Singapore 138668, Singapore. steve_oh@bti.a-star.edu.sg
Key Words Soy hydrolysate; Bovine serum albumin; Differentiation; Cardiomyocyte; Human embryonic stem cells; Human induced pluripotent stem cells
Core Tip Nutrient supplements were screened for improving cell survival during the cardiomyocyte differentiation process of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) in a serum-free medium based on the inhibition of p38 MAPK (SB media). Soy hydrolysate and bovine serum albumin supplementation was found to improve cell viability and therefore increased the yield of HES-3 cardiomyocytes by 2.6-fold over non-supplemented SB medium. The enhancing effect of this medium was demonstrated in an additional hESC line (H1) and Siu1-hiPSC cell line (15 and 9 fold respectively). The cardiomyocytes formed expressed canonical cardiac markers (sarcomeric ?-actinin, myosin heavy chain and troponin-T) and demonstrated all three major phenotypes: nodal-, atrial- and ventricular-like.
Publish Date 2013-07-25 08:11
Citation Ting S, Lecina M, Chan YC, Tse HF, Reuveny S, Oh SKW. Nutrient supplemented serum-free medium increases cardiomyogenesis efficiency of human pluripotent stem cells. World J Stem Cells 2013; 5(3): 86-97
URL http://www.wjgnet.com/1948-0210/full/v5/i3/86.htm
DOI http://dx.doi.org/10.4252/wjsc.v5.i3.86
Full Article (PDF) WJSC-5-86.pdf
Manuscript File 2977-Review.docx
Answering Reviewers 2977-Answering reviewers.docx
Copyright License Agreement 2977-Copyright assignment.PDF
Peer-review Report 2935-Peer review(s).pdf
Scientific Editor Work List 2935-Scientific editor work list.doc