ISSN |
1007-9327 (print) and 2219-2840 (online) |
Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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Copyright |
© The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
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Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
Website |
http://www.wjgnet.com |
Category |
Infectious Diseases |
Manuscript Type |
Basic Study |
Article Title |
Novel CagA ELISA exhibits enhanced sensitivity of Helicobacter pylori CagA antibody
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Manuscript Source |
Invited Manuscript |
All Author List |
Yuichi Matsuo, Yasutoshi Kido, Junko Akada, Seiji Shiota, Tran Thanh Binh, Tran Thi Huyen Trang, Ho DQ Dung, Pham Huu Tung, Tran Dinh Tri, Ngo P Minh Thuan, Le Quang Tam, Bui Chi Nam, Vu Van Khien and Yoshio Yamaoka |
Funding Agency and Grant Number |
Funding Agency |
Grant Number |
The National Institutes of Health |
DK62813 |
The Ministry of Education, Culture, Sports, Science, and Technology of Japan |
25293104 |
The Ministry of Education, Culture, Sports, Science, and Technology of Japan |
26640114 |
The Ministry of Education, Culture, Sports, Science, and Technology of Japan |
15H02657 |
The Japan Society for the Promotion of Science Institutional Program for Young Researcher Overseas Visits |
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The Strategic Funds for the Promotion of Science and Technology from the Japan Science and Technology Agency |
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Corresponding Author |
Yamaoka Yoshio, MD, PhD, Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Oita 879-5593,
Japan. yyamaoka@oita-u.ac.jp
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Key Words |
Helicobacter pylori; cagA genotype; Anti-CagA antibody; Enzyme-linked immunosorbent assay; Inflammation; Gastritis |
Core Tip |
We developed a novel East Asian-type CagA enzyme-linked immunosorbent assay (ELISA) to determine whether this method could detect CagA seropositivity with greater sensitivity in East Asian countries than the conventional anti-CagA antibody ELISA, which utilizes Western-type CagA as the antigen. Our findings revealed that conventional CagA ELISA underestimated CagA seropositivity in East Asian countries and the novel CagA ELISA could detect anti-CagA antibodies with higher sensitivity. In addition, the anti-CagA antibody titer tended to correlate with chronic inflammation in the stomach. Therefore, the titer of East Asian CagA ELISA may be a useful marker for predicting chronic inflammation in the gastric mucosa.
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Publish Date |
2017-01-05 11:10 |
Citation |
Matsuo Y, Kido Y, Akada J, Shiota S, Binh TT, Trang TTH, Dung HDQ, Tung PH, Tri TD, Thuan NPM, Tam LQ, Nam BC, Khien VV, Yamaoka Y. Novel CagA ELISA exhibits enhanced sensitivity of Helicobacter pylori CagA antibody. World J Gastroenterol 2017; 23(1): 48-59 |
URL |
http://www.wjgnet.com/1007-9327/full/v23/i1/48.htm |
DOI |
http://dx.doi.org/10.3748/wjg.v23.i1.48 |