| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. |
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| Permissions |
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Gastroenterology & Hepatology |
| Manuscript Type |
Basic Study |
| Article Title |
Faecal and mucosal microbiota in patients with functional gastrointestinal disorders: Correlation with toll-like receptor 2/toll-like receptor 4 expression
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Li-Na Dong, Jun-Ping Wang, Ping Liu, Yun-Feng Yang, Jing Feng and Yi Han |
| ORCID |
|
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| International Science and Technology Cooperation Project of Shanxi |
2013081066 |
| Science Foundation of Health and Family Planning Commission of Shanxi Province |
201201059 |
| Science Foundation of Health and Family Planning Commission of Shanxi Province |
201601014 |
|
| Corresponding Author |
Jun-Ping Wang, Professor, Department of Gastroenterology, Shanxi Provincial People’s Hospital, Affiliate of Shanxi Medical University, Shanxi Provincial People’s Hospital, 29 Shuangta Road, Taiyuan 030012, Shanxi Province, China. wangjp8396@sohu.com |
| Key Words |
Gastrointestinal microbiota; 16S rRNA gene; Toll-like receptors; High-throughput sequencing |
| Core Tip |
To explore which bacteria regulate the expression of toll-like receptors (TLRs) and thereby affect intestinal functions, we performed high-throughput pyrosequencing of the bacterial 16S rRNA gene, compared the microbial communities in the faeces and mucosa of Chinese patients with functional gastrointestinal disorders, and studied their association with the expression of colonic mucosal TLR2 and TLR4. Samples of luminal microbiota were different from those of mucosa-associated microbiota (MAM), and MAM samples were closely associated with TLR2/4 expression. The abundance of Faecallibacterium and Ruminococcus was lower in patients with gut disease, while the expression of TLRs is higher than in healthy controls. The presence of Faecallibacterium and Ruminococcus was significantly and negatively correlated with TLR4 expression, suggesting that these two bacteria, which colonize on the colonic mucosa, play a key role in gut diseases by regulating mucosal TLR4 expression. Granulicatella, which belongs to Carnobacteriaceae, and Streptococcus, which belongs to Streptococcaceae, were inversely correlated with TLR2 expression. Because the two genera contained not only pathogenic species but also probiotic species, it will be important to establish a better understanding of the relationship between TLRs and bacterial strains in future studies. |
| Publish Date |
2017-09-25 08:56 |
| Citation |
Dong LN, Wang JP, Liu P, Yang YF, Feng J, Han Y. Faecal and mucosal microbiota in patients with functional gastrointestinal disorders: Correlation with toll-like receptor 2/toll-like receptor 4 expression. World J Gastroenterol 2017; 23(36): 6665-6673 |
| URL |
http://www.wjgnet.com/1007-9327/full/v23/i36/6665.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v23.i36.6665 |
