| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Gastroenterology & Hepatology |
| Manuscript Type |
Basic Study |
| Article Title |
Analysis of hepatitis B virus preS1 variability and prevalence of the rs2296651 polymorphism in a Spanish population
|
| Manuscript Source |
Invited Manuscript |
| All Author List |
Rosario Casillas, David Tabernero, Josep Gregori, Irene Belmonte, Maria Francesca Cortese, Carolina González, Mar Riveiro-Barciela, Rosa Maria López, Josep Quer, Rafael Esteban, Maria Buti and Francisco Rodriguez-Frias |
| ORCID |
|
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Instituto de Salud Carlos II, cofinanced by the European Regional Development Fund (ERDF) |
PI14/01416 |
| Instituto de Salud Carlos II, cofinanced by the European Regional Development Fund (ERDF) |
PI15/00856 |
| Gilead Fellowship Program |
GLD14-00296 |
|
| Corresponding Author |
David Tabernero, PhD, Postdoc, Research Scientist, Liver Pathology Unit, Departments of Biochemistry and Microbiology, Hospital Universitari Vall d’Hebron, Universitat Autònoma de Barcelona, Passeig Vall d’Hebron 119-129, clinical laboratories, office 212, Barcelona 08035, Spain. david.tabernero@ciberehd.org |
| Key Words |
Hepatitis B virus; Hepatitis B virus preS1 region; Sodium-taurocholate co-transporting polypeptide; NTCP-interacting domain; Virion morphogenesis domain; SNP rs2296651; Next-generation sequencing; Real-time PCR melting curves |
| Core Tip |
Simultaneous analysis of both viral and host features important for hepatitis B virus (HBV) entry into hepatocytes provided locally relevant preliminary information in a population previously uncharacterized in this regard. In-house developed next-generation sequencing was successfully used to investigate the variability of the preS1 domain of the HBV large envelope protein, and real-time PCR melting curve analysis to detect the rs2296651 polymorphism (NTCP variant, S267F) in the HBV cellular receptor, NTCP. Results in a limited sample indicate that the features analyzed would not decrease the effectiveness of new therapies to block NTCP and avert HBV binding to hepatocytes in our particular CHB population. |
| Publish Date |
2018-02-06 03:22 |
| Citation |
Casillas R, Tabernero D, Gregori J, Belmonte I, Cortese MF, González C, Riveiro-Barciela M, López RM, Quer J, Esteban R, Buti M, Rodríguez-Frías F. Analysis of hepatitis B virus preS1 variability and prevalence of the rs2296651 polymorphism in a Spanish population. World J Gastroenterol 2018; 24(6): 680-692 |
| URL |
http://www.wjgnet.com/1007-9327/full/v24/i6/680.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v24.i6.680 |
