| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Virology |
| Manuscript Type |
Basic Study |
| Article Title |
Detection of hyper-conserved regions in hepatitis B virus X gene potentially useful for gene therapy
|
| Manuscript Source |
Invited Manuscript |
| All Author List |
Carolina González, David Tabernero, Maria Francesca Cortese, Josep Gregori, Rosario Casillas, Mar Riveiro-Barciela, Cristina Godoy, Sara Sopena, Ariadna Rando, Marçal Yll, Rosa Maria López, Josep Quer, Rafael Esteban, Maria Buti and Francisco Rodriguez-Frias |
| ORCID |
|
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Instituto de Salud Carlos III |
grant PI15/00856 |
| European Regional Development Fund (ERDF) |
grant PI15/00856 |
|
| Corresponding Author |
Maria Francesca Cortese, PhD, Postdoc, Research Scientist, Liver Pathology Unit, Departments of Biochemistry and Microbiology, Hospital Universitari Vall d’Hebron, Universitat Autònoma de Barcelona, Passeig Vall d’Hebron 119-129, clinical laboratories, office 212, Barcelona 08035, Spain. maria.cortese@vhir.org |
| Key Words |
Hepatitis B virus; Hepatitis B X gene; Hepatitis B X protein; Gene therapy; Next-generation sequencing; HBV conserved regions; Small interference RNA |
| Core Tip |
Hepatitis B virus (HBV) is not cured with classic treatments, and liver disease can progress by persistence and expression of covalently-closed circular DNA. Gene therapy with small interference RNA may be an effective approach to ensure inhibition of viral expression and disease progression, and hepatitis B virus X gene (HBX) transcripts could be optimal targets for this therapy. This study includes patients with different HBV genotypes and clinical stages to cover many clinical and virological situations. Using next-generation sequencing, we found two hyper-conserved HBX regions, candidates for small interference RNA therapy, which could enable pan-genotypic inhibition of HBV expression, regardless of the patients’ disease status. |
| Publish Date |
2018-05-18 02:03 |
| Citation |
González C, Tabernero D, Cortese MF, Gregori J, Casillas R, Riveiro-Barciela M, Godoy C, Sopena S, Rando A, Yll M, Lopez-Martinez R, Quer J, Esteban R, Buti M, Rodríguez-Frías F. Detection of hyper-conserved regions in hepatitis B virus X gene potentially useful for gene therapy. World J Gastroenterol 2018; 24(19): 2095-2107 |
| URL |
http://www.wjgnet.com/1007-9327/full/v24/i19/2095.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v24.i19.2095 |
