| Category |
Gastroenterology & Hepatology |
| Manuscript Type |
Autobiography |
| Article Title |
Epidermal growth factor upregulates Skp2/Cks1 and p27kip1 in human extrahepatic cholangiocarcinoma cells
|
| Manuscript Source |
Invited Manuscript |
| All Author List |
Ja-yeon Kim, Hong Joo Kim, Jung Ho Park, Dong Il Park, Yong Kyun Cho, Chong Il Sohn, Woo Kyu Jeon, Byung Ik Kim, Dong Hoon Kim, Seoung Wan Chae and Jin Hee Sohn |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Samsung Biomedical Research Institute |
C-A9-210-1 |
|
| Corresponding Author |
Hong Joo Kim, MD, Department of Internal Medicine, Sungkyunkwan University Kangbuk Samsung Hospital, 108, Pyung-Dong, Jongro-Ku, Seoul 110-746, South Korea. hongjoo3.kim@samsung.com |
| Key Words |
S-phase kinase-associated protein 2; Cyclin-dependent kinases regulatory subunit 1; P27kip1; Cholangiocarcinoma; E2F1; PI3K/Akt |
| Core Tip |
Based on the idea that S-phase kinase-associated protein 2 (Skp2) and p27kip1 might play a role in the pathogenesis and disease progression of patients with extrahepatic cholangiocarcinoma, immunohistochemical staining for Skp2, cyclin-dependent kinases regulatory subunit 1 (Cks1), p27kip1, and Ki67, along with other relevant molecular biologic experiments, were performed in tissue samples and human cholangiocarcinoma cell lines. Higher immunostaining intensity of Skp2 was an independent prognostic factor in patients with extrahepatic cholangiocarcinoma, and exogenous epidermal growth factor upregulates mRNA and protein levels of Skp2/Cks1 and p27kip1 in SNU-1196, SNU-1079, and SNU-245 cells. By chromatin immunoprecipitation assay, we found that E2F1 transcription factor directly binds to the promoter site of Skp2. |
| Publish Date |
2014-02-19 16:57 |
| Citation |
Kim J, Kim HJ, Park JH, Park DI, Cho YK, Sohn CI, Jeon WK, Kim BI, Kim DH, Chae SW, Sohn JH. Epidermal growth factor upregulates Skp2/Cks1 and p27kip1 in human extrahepatic cholangiocarcinoma cells. World J Gastroenterol 2014; 20(3): 755-773 |
| URL |
http://www.wjgnet.com/1007-9327/full/v20/i3/755.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v20.i3.755 |
