Publication Name	World Journal of Gastroenterology
Manuscript ID	4721
Country

Received	2013-07-19 09:27
Peer-Review Started	2013-07-19 22:03
To Make the First Decision	2013-09-02 16:27
Return for Revision	2013-09-03 21:29
Revised	2013-11-29 09:37
Second Decision	2014-01-08 18:06
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2014-01-08 18:44
Articles in Press
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2014-05-20 11:57
Publish the Manuscript Online	2014-06-06 16:55

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access
Copyright
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Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Gastroenterology & Hepatology
Manuscript Type	Brief Articles
Article Title	Dual-priming oligonucleotide-based multiplex PCR using tissue samples in rapid urease test in the detection of Helicobacter pylori infection
Manuscript Source
All Author List	Woo Chul Chung, Sung Hoon Jung, Jung Hwan Oh, Tae Ho Kim, Dae Young Cheung, Byung Wook Kim, Sung Soo Kim, Jin Il Kim and Eun Young Sin
Funding Agency and Grant Number
Corresponding Author	Sung Hoon Jung, MD, Department of Internal Medicine, the Catholic University of Korea, St. Paul’s Hospital, 180 Wangsan-ro, Dongdaemun-gu, Seoul 130-709, South Korea. shjung74@catholic.ac.kr
Key Words	Helicobacter pylori; Diagnosis; Dual-priming oligonucleotide-based multiplex polymerase chain reaction
Core Tip	The rapid urease test (CLOtest) alone is unreliable in diagnosing Helicobacter pylori (H. pylori) infection and does not provide information about resistance to clarithromycin. Therefore, we investigated whether tissue samples that have been analyzed by the CLOtest kit are suitable for dual-priming oligonucleotide-based multiplex PCR (DPO-PCR) to detect H. pylori. Our results demonstrated that the DPO-based multiplex PCR test using tissue samples processed by the CLO kit is appropriate for detecting H. pylori and clarithromycin resistance. Particularly, in patients with CLO-negative results, this method is helpful for diagnosing H. pylori infection. Moreover, it would be beneficial in economical aspects.
Publish Date	2014-06-06 16:55
Citation	Chung WC, Jung SH, Oh JH, Kim TH, Cheung DY, Kim BW, Kim SS, Kim JI, Sin EY. Dual-priming oligonucleotide-based multiplex PCR using tissue samples in rapid urease test in the detection of Helicobacter pylori infection. World J Gastroenterol 2014; 20(21): 6547-6553
URL	http://www.wjgnet.com/1007-9327/full/v20/i21/6547.htm
DOI	http://dx.doi.org/10.3748/wjg.v20.i21.6547

Full Article (PDF)	WJG-20-6547.pdf
Full Article (Word)	WJG-20-6547.doc
Manuscript File	4721-Review.docx
Answering Reviewers	4721-Answering reviewers.pdf
Copyright License Agreement	4721-Copyright assignment.pdf
Non-Native Speakers of English Editing Certificate	4721-Language certificate.pdf
Peer-review Report	4721-Peer review(s).pdf
Scientific Editor Work List	4721-Scientific editor work list.doc