| ISSN |
1948-0210 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Transplantation |
| Manuscript Type |
Basic Study |
| Article Title |
How old is too old? In vivo engraftment of human peripheral blood stem cells cryopreserved for up to 18 years - implications for clinical transplantation and stability programs
|
| Manuscript Source |
Invited Manuscript |
| All Author List |
John Underwood, Mahvish Rahim, Carijo West, Rebecca Britton, Elaine Skipworth, Vicki Graves, Steven Sexton, Hillary Harris, Dave Schwering, Anthony Sinn, Karen E Pollok, Kent A Robertson, W Scott Goebel and Kerry M Hege |
| ORCID |
|
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| NIH/NIDDK |
P30DK090948 |
| NIH/NCI |
P30CA082709 |
|
| Corresponding Author |
W Scott Goebel, MD, PhD, Associate Professor, Division of Pediatric Hematology/Oncology, Indiana University School of Medicine, Room 2626, 705 Riley Hospital Drive, Indianapolis, IN 46202, United States. sgoebel2@iu.edu |
| Key Words |
Colony-forming units assay; Cryopreservation; Hematopoietic stem cells; Hematopoietic stem cell transplantation; In vitro techniques; Peripheral blood stem cell; Viability; Transplant; Long-term storage |
| Core Tip |
Peripheral blood stem cells (PBSC) are commonly cryopreserved awaiting clinical use for hematopoietic stem cell transplant. Long term cryopreservation is commonly defined as five years or longer, and limited data exists regarding how long PBSC can be cryopreserved and retain the ability to successfully engraft. We demonstrated engraftment of clinically-collected and thawed PBSC following cryopreservation up to 18 years in NSG mice, signifying likely successful clinical transplantation of PBSC following long-term cryopreservation. |
| Publish Date |
2020-05-26 14:31 |
| Citation |
Underwood J, Rahim M, West C, Britton R, Skipworth E, Graves V, Sexton S, Harris H, Schwering D, Sinn A, Pollok KE, Robertson KA, Goebel WS, Hege KM. How old is too old? In vivo engraftment of human peripheral blood stem cells cryopreserved for up to 18 years - implications for clinical transplantation and stability programs. World J Stem Cells 2020; 12(5): 359-367 |
| URL |
https://www.wjgnet.com/1948-0210/full/v12/i5/359.htm |
| DOI |
https://dx.doi.org/10.4252/wjsc.v12.i5.359 |
