| ISSN |
1948-0210 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Cell Biology |
| Manuscript Type |
Basic Study |
| Article Title |
Enrichment of retinal ganglion and Müller glia progenitors from retinal organoids derived from human induced pluripotent stem cells - possibilities and current limitations
|
| Manuscript Source |
Invited Manuscript |
| All Author List |
Kristine Karla Freude, Sarkis Saruhanian, Alanna McCauley, Colton Paterson, Madeleine Odette, Annika Oostenink, Poul Hyttel, Mark Gillies, Henriette Haukedal and Miriam Kolko |
| ORCID |
|
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Innovation Fund Denmark |
4108-00008B |
| The Bagenkop Nielsens Øjen-Fond |
115227 |
| Hørslev-Fonden |
116967 |
| Beckett Fonden |
116936 |
| Velux Foundation |
1179261001/2 |
|
| Corresponding Author |
Kristine Karla Freude, BSc, DPhil, MSc, PhD, Associate Professor, Department of Veterinary and Animal Sciences, Group of Stem Cell Models for Studies of Neurodegenerative Diseases, Section for Pathobiological Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 7, Frederiksberg 1870, Denmark. kkf@sund.ku.dk |
| Key Words |
Human induced pluripotent stem cells; Retinal organoids; Retinal ganglion cells; Müller glia; Progenitors; Cell-type enrichment |
| Core Tip |
Retinal organoids derived from human induced pluripotent stem cells present excellent tools to enrich specific subpopulations for subsequent studies of cell-type specific disease phenotypes affecting the eye. Here we describe the generation of retinal organoids and the harvest as well as 2D maintenance of retinal ganglion cells and Müller glia progenitors. |
| Publish Date |
2020-10-26 12:01 |
| Citation |
Freude KK, Saruhanian S, McCauley A, Paterson C, Odette M, Oostenink A, Hyttel P, Gillies M, Haukedal H, Kolko M. Enrichment of retinal ganglion and Müller glia progenitors from retinal organoids derived from human induced pluripotent stem cells - possibilities and current limitations. World J Stem Cells 2020; 12(10): 1171-1183 |
| URL |
https://www.wjgnet.com/1948-0210/full/v12/i10/1171.htm |
| DOI |
https://dx.doi.org/10.4252/wjsc.v12.i10.1171 |
