Publication Name	World Journal of Stem Cells
Manuscript ID	59244
Country	China

Received	2020-09-01 03:05
Peer-Review Started	2020-09-01 03:05
To Make the First Decision
Return for Revision	2020-09-15 22:35
Revised	2020-11-01 01:10
Second Decision	2020-11-17 12:25
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2020-11-17 22:54
Articles in Press	2020-11-17 22:54
Publication Fee Transferred
Edit the Manuscript by Language Editor	2020-11-30 23:54
Typeset the Manuscript	2021-01-18 06:21
Publish the Manuscript Online	2021-01-21 03:12

ISSN	1948-0210 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
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Category	Cell Biology
Manuscript Type	Basic Study
Article Title	Transcriptomic alterations underline aging of osteogenic bone marrow stromal cells
Manuscript Source	Invited Manuscript
All Author List	Yu-Hao Cheng, Shu-Fen Liu, Jing-Cheng Dong and Qin Bian
ORCID	Author(s) ORCID Number Yu-Hao Cheng http://orcid.org/0000-0002-7472-8518 Shu-Fen Liu http://orcid.org/0000-0001-5037-2169 Jing-Cheng Dong http://orcid.org/0000-0002-3645-0324 Qin Bian http://orcid.org/0000-0003-4190-0913
Funding Agency and Grant Number	Funding Agency Grant Number National Natural Science Foundation of China 81573992
Corresponding Author	Qin Bian, MD, PhD, Professor, Department of Integrative Medicine, Huashan Hospital, Fudan University, Middle Wulumuqi Rd, Shanghai 200040, China. bianqin213@126.com
Key Words	Bone marrow stromal cell; Mesenchymal stem cell; Mesenchymal stromal cell; Aging; Bone modeling and remodeling; Transcriptome
Core Tip	Multipotent bone marrow stromal cells (BMSCs) are adult stem cells that form functional osteoblasts and play a critical role in bone remodeling. During aging, an increase in bone loss and reduction in structural integrity lead to osteoporosis and result in an increased risk of fracture. In this study, we examined age-dependent histological changes in murine vertebrae and uncovered that bone loss begins as early as the age of one month. The BMSCs isolated at different ages revealed a consistent decreasing trend in both colony-forming and osteogenic commitment capacity. Moreover, we obtained the transcriptomic profiles of BMSCs at 1 mo, 3 mo, and 7 mo to investigate the distinct molecular and regulatory features underpin the early loss of osteogenic potential. We showed the enrichment of osteoblastic regulation genes at one month and loss of osteogenic features at three months. The adipogenic and DNA repair features were enriched in the later age at seven months. Moreover, we demonstrated that the WNT and MAPK signaling pathways were upregulated at one month, followed by increased pro-inflammatory and apoptotic features.
Publish Date	2021-01-21 03:12
Citation	Cheng YH, Liu S, Dong JC, Bian Q. Transcriptomic alterations underline aging of osteogenic bone marrow stromal cells. World J Stem Cells 2021; 13(1): 128-138
URL	https://www.wjgnet.com/1948-0210/full/v13/i1/128.htm
DOI	https://dx.doi.org/10.4252/wjsc.v13.i1.128

Full Article (PDF)	WJSC-13-128.pdf
Full Article (Word)	WJSC-13-128.docx
Manuscript File	59244_Auto_Edited_WangTQ.docx
Answering Reviewers	59244-Answering reviewers.pdf
Audio Core Tip	59244-Audio core tip.m4a
Biostatistics Review Certificate	59244-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	59244-Conflict-of-interest statement.pdf
Copyright License Agreement	59244-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	59244-Grant application form(s).pdf
Institutional Animal Care and Use Committee Approval Form or Document	59244-Institutional animal care and use committee statement.pdf
Peer-review Report	59244-Peer-review(s).pdf
Scientific Misconduct Check	59244-Scientific misconduct check.pdf
Scientific Editor Work List	59244-Scientific editor work list.pdf